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020 _a9781592594818
024 7 _a10.1385/0896033864
_2doi
040 _aES-MaUEC
_bspa
_cES-MaUEC
_dES-MaUEC
050 4 _aTP248.65.I45
_b1997 EB
245 0 0 _aImmobilization of Enzymes and Cells
_cedited by Gordon Bickerstaff
250 _a1st edition 1997
264 1 _aTotowa, NJ
_bHumana Press
_c1997
300 _a1 recurso en línea (XIV, 367 páginas)
336 _atexto
_btxt
_2rdacontent
337 _aelectrónico
_bc
_2rdamedia
338 _arecurso electrónico
_bcr
_2rdacarrier
347 _aarchivo de texto
_bPDF
490 0 _aMethods in Biotechnology
_x1940-607X
_v1
505 0 _aImmobilization of Enzymes and Cells -- Immobilization of Enzymes by Selective Adsorption on Biotinylaminopropyl Celite or Glass -- Immobilization of Proteins on Thionyl Chloride-Activated Controlled-Pore Glass -- Enzyme Immobilization on Nylon -- Visual Assessment of Enzyme Immobilization -- Immobilization in Carrageenans -- Entrapment in Calcium Alginate -- Entrapment of Enzymes and Cells in Poly (2-Hydroxyethyl Methacrylate) Supports -- Activation of Rayon/Polyester Cloth for Protein Immobilization -- Immobilization of Enzymes on Microelectrodes Using Chemical Crosslinking -- Photolithographic Patterning of Enzyme Membranes for the Modification of Microelectrodes -- Electrochemical-Based Immobilization of Enzymes -- Immobilization of Enzymes on Thermo-Responsive Polymers -- Immobilization of Photosynthetic Membranes in an Albumin-Glutaraldehyde Crosslinked Matrix -- Poly(Ethylene Glycol) Crosslinked to Albumin as a Support for Enzyme Immobilization -- Poly(Carbamoyl Sulfonate) Hydrogels -- Enzyme Immobilization on Polyethyleneimine-Coated Magnetite Particles -- Immobilization of Enzymes and Proteins on Red Blood Cells -- Cellulose Paper Support for Immobilization -- Immobilization of Cells Using Electrostatic Droplet Generation -- Hepatocyte Immobilization in Agarose and Functional Integrity Testing -- Immobilized Hepatocytes in Xenobiotic Biotransformation Studies -- Immobilization of Liposomes and Proteoliposomes in Gel Beads -- Cell Immobilization with Phosphorylated Polyvinyl Alcohol (PVA) Gel -- Covalent Immobilization of Enzymes to Graphitic Particles -- Enzyme Immobilization Using Chitosan-Xanthan Complexes -- Calcium Alginate Film Formed on a Stainless Steel Mesh -- Preparation of Immobilized Subunits of a Multisubunit Enzyme -- Characterization of Enzyme Activity, Protein Content, and Thiol Groups in Immobilized Enzymes -- Immobilization of Enzymes Acting on Macromolecular Substrates -- Immobilization of Enzymes on Glyoxyl Agarose -- Stabilization of Immobilized Enzymes by Chemical Modification with Polyfunctional Macromolecules -- Covalent Immobilization of Enzymes Using Commercially Available CDI-Activated Agarose -- Immobilization of Cells in Polyelectrolyte Complexes -- Coimmobilization of Enzymes and Cells -- Adsorption of Lipase on Inorganic Supports -- Immobilization of Enzymes on Inorganic Supports by Covalent Methods -- Use of Divalent Metal Ions Chelated to Agarose Derivatives for Reversible Immobilization of Proteins -- Transition Metal Methods for Immobilization of Enzymes and Cells.
520 _aImmobihzatron of enzymes, cells, and organelles has expanded greatly in the past 30 years as the advantages of immobilization have been evaluated and utilized in analyttcal, biotransformation, and medical applications. A c- sequence of this explosion of technology IS that there is now a bewildering array of permutations for the immobilization of biological material. The p- pose of Immobilization of Enzymes and Cells is to provrde a basic reference tool for all academic and industrial research workers seeking to start or expand the use of mnnobilization techniques in their work. The book does not aim to provide comprehensive coverage of the vast range of methods available, but will serve as a launch pad for potential users of immobilization techniques. One reason for the vast expanse of mmrobilization technology lies m the subject material to be immobilized. Biological catalysts (enzymes, organelles, and cells) have a high degree of individual variability, and although many tmmobilization techniques have wide applicability, tt is imposstble for one or even a few methods to cater to the great diversity of requirements inherent in biological material. This is especially so when the atm is to produce an op- mum system m which the immobihzed biocatalyst will function at high levels of efficrency, stability, and so on.
988 _aSpringer_Protocols_1997
650 7 _2embne
_9138640
_aEnzimas
650 7 _2embne
_9140931
_aBiotecnología
776 0 8 _iPrinted edition:
_z9781489941343
776 0 8 _iPrinted edition:
_z9781489941336
776 0 8 _iPrinted edition:
_z9780896033863
856 4 0 _uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1385/0896033864
_zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
942 _2lcc
_cLE
998 _b06/2023
_dz
_eIG
_zSI