000			04989nam a22003975i 4500
999			_c391933 _d391933
001			391933
003			ES-MaUEC
005			20230615134745.0
006			a|||| o|||| 00| 0
007			cr nn 008mamaa
008			230615s1998 xxu| o |||| 0|eng d
020			_a9781592595631
024	7		_a10.1385/089603397X _2doi
040			_aES-MaUEC _bspa _cES-MaUEC _dES-MaUEC
050		4	_aQP551 _b1998 EB
245	0	0	_aProtein Synthesis : _bMethods and Protocols _cedited by Robin Martin
250			_a1st edition 1998
264		1	_aTotowa, NJ _bHumana Press _c1998
300			_a1 recurso en línea (XIV, 442 páginas)
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v77
505	0		_aAnalyses of Ribosome Distribution During In Vitro Translation -- Allele-Specific Priming in the Mapping of rRNA -- Permeabilized Mammalian Cells as a System for Protein Synthesis -- The Measurement of Processivity Errors in Protein Synthesis -- A Biosynthetic Approach for the Incorporation of Unnatural Amino Acids into Proteins -- The Analysis of Translational Activity Using a Reporter Gene Constructed from Repeats of an Antibody-Binding Domain from Protein A -- In Vitro Engineering Using Acyl-Derivatized tRNAs -- In Vitro Engineering Using Synthetic tRNAs with Altered Anticodons Including Four-Nucleotide Anticodons -- Analysis of rRNA Function Using Specialized Ribosomes -- Determination of the Peptide Elongation Rate In Vivo -- The Measurement of Missense Errors During In Vivo Protein Synthesis -- Heelprinting Analysis of In Vivo Ribosome Pause Sites -- Analysis of elF-2? Kinases in Yeast -- Continuous-Flow Cell-Free Translation, Transcription-Translation, and Replication-Translation Systems -- Analysis of Translational Activity of Extracts Derived from Oocytes and Eggs of Xenopus laevis -- A Fractionated Reticulocyte Lysate System for Studies on Protein Synthesis Initiation Factors -- Measurement of Ribosomal Accuracy and Proofreading in E. coli Burst Systems -- Measurement of Rate of Protein Synthesis In Vitro -- In Vivo Mutational Analysis of Ribosomal RNA in Saccharomyces cerevisiae -- Genetic Selection of rRNA Mutations -- Toeprinting Assays -- Mapping of Pseudouridine Residues in RNA to Nucleotide Resolution -- Chemical and Enzymatic Probing of Antibiotic-Ribosome Complexes -- Protein Engineering with Nonstandard Amino Acids -- Internal Ribosome Entry Sites Tests with Circular mRNAs -- Gel Retardation and UV-Crosslinking Assays to Detect Specific RNA-Protein Interactions in the 5? or 3? UTRs of Translationally Regulated mRNAs -- Translational Control by Repressor Proteins Binding to the 5?UTR of mRNAs -- Identification and Analysis of Frameshift Sites -- Synthesis and Site-Specific Binding of Thioated tRNAs to Probe Ribosome-tRNA Interactions.
520			_aThe synthesis of proteins from 20 or so constituent amino acids according to a strictly defined code with an accuracy of better than 1 in 10,000 at most loca­ tions is arguably the most complex task performed by cells. Protein Synthesis collects together methods and protocols covering a range of different approaches towards understanding how the cellular machinery accomplishes this task and how these ftinctions might be harnessed by the biotechnology industry to generate novel and useful proteins. The era in which the components of the translational machinery were being catalogued is over. This volume gathers together protocols that focus on preserving and describing the dynamic function as closely as possible. The need to understand exactly how ribosomes are positioned on messages or where tRNA molecules, translation factors, or control proteins are bound, has been appreciated by many of the authors. Several chapters that explore the fidelity and processivity of translation reflect this belief. Moreover, the fundamental importance of rRNA at the heart of the ribosome is a strong theme in a number of the protocols. These articles include in vitro and in vivo systems from bacterial, fungal, plant, and animal systems. Overall, Protein Synthesis might be characterized by the novelty of the approaches employed to illuminate the inner workings of the protein synthetic machinery as well as by the inventiveness of the attempts to harness these reactions for biotechnological applications.
988			_aSpringer_Protocols_1998
650		7	_2embne _9139861 _aProteínas
776	0	8	_iPrinted edition: _z9781617370250
776	0	8	_iPrinted edition: _z9780896033979
776	0	8	_iPrinted edition: _z9781489942746
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1385/089603397X _zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
942			_2lcc _cLE
998			_b06/2023 _dz _eIG _zSI