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020 _a9781493973378
024 7 _a10.1007/978-1-4939-7337-8
_2doi
040 _aES-MaUEC
_bspa
_cES-MaUEC
_dES-MaUEC
050 4 _aSB191 .W5
_b2017 EB
245 0 0 _aWheat Biotechnology :
_bMethods and Protocols
_cedited by Prem L. Bhalla, Mohan B. Singh
250 _a1st edition 2017
264 1 _aNew York, NY
_bSpringer International Publishing
_c2017
300 _a1 recurso en línea (XI, 309 páginas)
_b53 ilustraciones, 50 ilustraciones a color
336 _atexto
_btxt
_2rdacontent
337 _aelectrónico
_bc
_2rdamedia
338 _arecurso electrónico
_bcr
_2rdacarrier
347 _aarchivo de texto
_bPDF
490 0 _aMethods in Molecular Biology
_x1940-6029
_v1679
505 0 _aEnabling Molecular Technologies for Trait Improvement in Wheat -- What Will Be the Benefits of Biotech Wheat for European Agriculture? -- Overview of the Wheat Genetic Transformation and Breeding Status in China -- Wheat Improvement in India: Present and Future -- Overview of Methods for Assessing Salinity and Drought Tolerance of Transgenic Wheat Lines -- Allergenicity Assessment of Transgenic Wheat Lines In Silico -- Agribusiness Perspectives on Transgenic Wheat -- Agrobacterium-Mediated Transformation of Wheat Using Immature Embryos -- Biolistic Transformation of Wheat -- Wheat Genetic Transformation Using Mature Embryos as Explants -- Targeted Mutagenesis in Hexaploid Bread Wheat Using the TALEN and CRISPR/Cas Systems -- Design and Assembly of CRISPR/Cas9 Reagents for Gene Knock-Out, Targeted Insertion, and Replacement in Wheat -- Doubled Haploid Transgenic Wheat Lines by Microspore Transformation -- Doubled Haploid Laboratory Protocol for Wheat Using Wheat-Maize Wide Hybridization -- Real-Time PCR for the Detection of Precise Transgene Copy Number in Wheat -- Endogenous Reference Genes and Their Quantitative Real-Time PCR Assays for Genetically Modified Bread Wheat (Triticum aestivum L.) Detection -- Phenotypic Characterization of Transgenic Wheat Lines Against Fungal Pathogens Puccinia triticina and Fusarium graminearum -- Databases for Wheat Genomics and Crop Improvement -- High Density SNP Genotyping Array for Hexaploid Wheat and Its Relatives.
520 _aThis detailed volume provides background on recent new technology developments highlighting the potential of the genomic era in wheat breeding with invaluable instruction on the methodology, which is complemented by overview chapters on the status of new technology application in major wheat production countries. The topics, addressed by internationally renowned scientists active in the field, cover methods underpinning the latest developments in the field of wheat biotechnology. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Wheat Biotechnology: Methods and Protocols serves as a vital resource for scientists working to breed future high-yielding wheat varieties to sustain a growing population in an increasingly unpredictable world.
988 _aSpringer_Protocols_2017
650 7 _2embne
_9140379
_aTrigo
_xBiotecnología
650 7 _2embne
_9667619
_aPlantas
_xMejora genética
776 0 8 _iPrinted edition:
_z9781493973354
776 0 8 _iPrinted edition:
_z9781493973361
776 0 8 _iPrinted edition:
_z9781493984565
856 4 0 _uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-4939-7337-8
_zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
942 _2lcc
_cLE
998 _b06/2023
_dz
_eIG
_zSI