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007			cr nn 008mamaa
008			230615s2009 xxu| o |||| 0|eng d
020			_a9781597454094
024	7		_a10.1007/978-1-59745-409-4 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC _dES-MaUEC
050		4	_aQP92 _b2009 EB
245	0	0	_aGenetic Modification of Hematopoietic Stem Cells : _bMethods and Protocols _cedited by Christopher Baum
250			_a1st edition 2009
264		1	_aTotowa, NJ _bHumana Press _c2009
300			_a1 recurso en línea (XXII, 490 páginas) _b84 ilustraciones, 10 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v506
505	0		_aImmunomagnetic Enrichment of Human and Mouse Hematopoietic Stem Cells for Gene Therapy Applications -- Isolation of Human and Mouse Hematopoietic Stem Cells -- Murine Hematopoietic Stem Cell Transduction Using Retroviral Vectors -- Genetic Modification of Human Hematopoietic Cells: Preclinical Optimization of Oncoretroviral-mediated Gene Transfer for Clinical Trials -- Short-Term Culture of Human CD34+ Cells for Lentiviral Gene Transfer -- T Cell Culture for Gammaretroviral Transfer -- Retroviral Transduction of Murine Primary T Lymphocytes -- Lentiviral Vector Gene Transfer into Human T Cells -- DNA Transposons for Modification of Human Primary T Lymphocytes -- Retroviral Gene Transfer into Primary Human Natural Killer Cells -- Lentiviral Vector-Mediated Genetic Programming of Mouse and Human Dendritic Cells -- In Situ (In Vivo) Gene Transfer into Murine Bone Marrow Stem Cells -- In Vivo and Ex Vivo Gene Transfer in Thymocytes and Thymocyte Precursors -- Design and Production of Retro- and Lentiviral Vectors for Gene Expression in Hematopoietic Cells -- Knock-Down of Gene Expression in Hematopoietic Cells -- The Use of Retroviral Vectors for tet-Regulated Gene Expression in Cell Populations -- Detection of Replication Competent Retrovirus and Lentivirus -- Release Testing of Retroviral Vectors and Gene-Modified Cells -- Copy Number Determination of Genetically-Modified Hematopoietic Stem Cells -- Tissue Procurement for Molecular Studies Using Laser-Assisted Microdissection -- Leukemia Diagnosis in Murine Bone Marrow Transplantation Models -- Humanized Mouse Models to Study the Human Haematopoietic Stem Cell Compartment -- Canine Models of Gene-Modified Hematopoiesis -- Detection of Retroviral Integration Sites by Linear Amplification-Mediated PCR and Tracking of Individual Integration Clones in Different Samples -- Retroviral Insertion Site Analysis in Dominant Haematopoietic Clones -- Tracking Gene-Modified T Cells In Vivo -- DNA Microarray Studies of Hematopoietic Subpopulations -- Quantification of Genomic Mutations in Murine Hematopoietic Cells -- Proteomics Studies After Hematopoietic Stem Cell Transplantation -- Spectral Karyotyping and Fluorescence In Situ Hybridization of Murine Cells -- Database Setup for Preclinical Studies of Gene-Modified Hematopoiesis -- The US and EU Regulatory Perspectives on the Clinical Use of Hematopoietic Stem/Progenitor Cells Genetically Modified Ex Vivo by Retroviral Vectors.
520			_aWith the incredible potential of gene transfer into hematopoietic stem cells, active research in this field has become critically important. In Genetic Modification of Hematopoietic Stem Cells: Methods and Protocols, leading scientists in the field provide a compendium of protocols which cover the subject comprehensively, from the purification and culture of various types of hematopoietic cells for subsequent genetic modification by vector development and technical issues of small and large scale vector production, to the complex issue of monitoring and biosafety studies related to gene-modified hematopoiesis. Written in the highly successful Methods in Molecular Biology™ series format, the chapters in this volume present brief introductions to the topic, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and Notes sections, which allow the experts to highlight tips on troubleshooting and avoiding known pitfalls. Unique and cutting-edge, Genetic Modification of Hematopoietic Stem Cells: Methods and Protocols is an ideal, thorough resource to promote further research and the implementation of investigator-driven clinical studies using gene-modified hematopoietic cells.
988			_aSpringer_Protocols_2009
650		7	_2embne _9165039 _aCélulas madre hematopoyéticas
650		7	_2embne _9141520 _aIngeniería genética
776	0	8	_iPrinted edition: _z9781607611172
776	0	8	_iPrinted edition: _z9781617378669
776	0	8	_iPrinted edition: _z9781588299802
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-59745-409-4 _zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
942			_2lcc _cLE
998			_b06/2023 _dz _eIG _zSI