000			04605nam a22004095i 4500
999			_c391923 _d391923
001			391923
003			ES-MaUEC
005			20230614083617.0
006			a|||| o|||| 00| 0
007			cr nn 008mamaa
008			230614s2017 xxu| s |||| 0|eng d
020			_a9781493966493
024	7		_a10.1007/978-1-4939-6649-3 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC _dES-MaUEC
050		4	_aQP551 _b2017 EB
245	0	0	_aType 3 Secretion Systems : _bMethods and Protocols _cedited by Matthew L. Nilles, Danielle L. Jessen Condry
250			_a1st edition 2017
264		1	_aNew York, NY _bSpringer International Publishing _c2017
300			_a1 recurso en línea (X, 224 páginas) _b32 ilustraciones, 23 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v1531
505	0		_aIntroduction to Type III Secretion Systems -- Site-Directed Mutagenesis and its Application in Studying the Interactions of T3S Components -- Blue Native Protein Electrophoresis to Study the T3S System using Yersinia pestis as a Model -- In Vivo Photo-Cross-Linking to Study T3S Interactions Demonstrated using the Yersinia pestis T3S System -- Isolation of Type III Secretion System Needle Complexes by Shearing -- Use of Transcriptional Control to Increase Expression and Secretion of Heterologous Proteins in T3S Systems -- Characterization of Type Three Secretion System Translocator Interactions with Phospholipid Membranes -- Analysis of Type III Secretion System Secreted Proteins -- Fractionation Techniques to Examine Effector Translocation -- Measurement of Effector Protein Translocation using Phosphorylatable Epitope Tags and Phospho-Specific Antibodies -- A TAL-Based Reporter Assay for Monitoring Type III-Dependent Protein Translocation in Xanthomonas -- Subcellular Localization of Pseudomonas syringae pv. tomato Effector Proteins in Plants -- A Method for Characterizing the Type III Secretion System's Contribution to Pathogenesis: Homologous Recombination to Generate Yersinia pestis Type III Secretion System Mutants -- Detecting Immune Responses to Type III Secretion Systems -- Recombinant Expression and Purification of the Shigella Translocator IpaB -- Expression and Purification of N-Terminally His-Tagged Recombinant Type III Secretion Proteins -- Mouse Immunization with Purified Needle Proteins from Type III Secretion Systems and the Characterization of the Immune Response to these Proteins -- Identification of the Targets of Type III Secretion System Inhibitors -- Detection of Protein Interactions in T3S Systems using Yeast 2-Hybrid Analysis.
520			_aThis volume discusses various basic and advanced methods and protocols that have been proven to be successful among certain bacterial species, or a family of species, in type III secretion systems (T3S system). The chapters in this book cover topics such as: site-directed mutagenesis and its application in studying the interactions of T3S components; use of transcriptional control to increase expression and secretion of heterologous proteins in T3S systems; fractionation techniques to examine effector translocation; detecting immune responses to T3S systems; mouse immunization with purified needle proteins from T3S systems and the characterization of the immune responses to these proteins; and detection of protein interactions in T3S systems using yeast 2-hybrid analysis. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Practical and comprehensive, Type 3 Secretion Systems: Methods and Protocols is a valuable resource for anyone interested in learning about the fascinating and ever-changing T3S systems.
988			_aSpringer_Protocols_2017
650		7	_2embne _9139861 _aProteínas
650		7	_2embne _9156720 _aMutagénesis
776	0	8	_iPrinted edition: _z9781493966479
776	0	8	_iPrinted edition: _z9781493966486
776	0	8	_iPrinted edition: _z9781493982608
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-4939-6649-3 _zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
942			_2lcc _cLE
998			_b06/2023 _dz _eIG _zSI