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008			230606s2011 xxu| s |||| 0|eng d
020			_a9781607619253
024	7		_a10.1007/978-1-60761-925-3 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC _dES-MaUEC
050		4	_aQK882 _b2011 EB
245	0	0	_aPhotosynthesis Research Protocols _cedited by Robert Carpentier
250			_a2nd edition 2011
264		1	_aTotowa, NJ _bHumana Press _c2011
300			_a1 recurso en línea (XIV, 395 páginas) _b71 ilustraciones
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v684
505	0		_aIsolation of Photosystem II-Enriched Membranes and the Oxygen-Evolving Complex (OEC) Subunit Proteins from Higher Plants -- Isolation of Photosystem I Submembrane Fractions -- Isolation of Photosystem II Reaction Center Complexes from Plants -- Methods for the Isolation of Functional Photosystem II Core Particles from the Cyanobacterium Synechocystis sp. PCC 6803 -- Purification and Crystallization of Oxygen-Evolving Photosystem II Core Complex from Thermophilic Cyanobacteria -- Isolation of Cytochrome b6f Complex from Grana and Stroma Membranes from Spinach Chloroplasts -- Purification and Crystallization of the Cytochrome b6f Complex -- Purification of Plastocyanin and Cytochrome c6 from Plants, Green Algae, and Cyanobacteria -- Isolation and Identification of Chloroplast Lipids -- Isolation and Purification of CP43 and CP47 Photosystem II Proximal Antenna Complexes from Plants -- Preparation of Native and Recombinant Light-Harvesting Chlorophyll-a/b Complex -- Isolation and Characterization of Lamellar Aggregates of LHCII and LHCII-Lipid Macro-Assemblies with Light-Inducible Structural Transitions -- Protein Targeting across and into Chloroplast Membranes -- Proteomic Analysis of Thylakoid Membranes -- Thylakoid Phosphoproteins: Identification of Phosphorylation Sites -- Direct Detection of Free Radicals and Reactive Oxygen Species in Thylakoids -- Assay of Photoinhibition and Heat Inhibition of Photosystem II in Higher Plants -- Photosystem II Reconstitution into Proteoliposomes and Methodologies for Structure-Function Characterization -- Physical and Chemical Immobilization Methods of Photosynthetic Materials -- Identifying Chloroplast Biogenesis and Signalling Mutants in Arabidopsis thaliana -- Expression of Genes in Cyanobacteria: Adaptation of Endogenous Plasmids as Platforms for High-Level Gene Expression in Synechococcus sp. PCC 7002 -- Construction of Gene Interruptions and Gene Deletions in the Cyanobacterium Synechocystis sp. Strain PCC 6803 -- A Simple Method for Chloroplast Transformation in Chlamydomonas reinhardtii -- Rapid Isolation of Intact Chloroplasts from Spinach Leaves -- Mechanical Isolation of Bundle Sheath Cell Strands and Thylakoids from Leaves of C4 Grasses -- Isolation of Ribulose-1,5-Bisphosphate Carboxylase/Oxygenase from Leaves -- Quantifying the Amount and Activity of Rubisco in Leaves -- Purification of Rubisco Activase from Leaves or after Expression in Escherichia coli -- Rubisco Activase Activity Assays -- Quantification of Rubisco Activase Content in Leaf Extracts.
520			_aThe capture of sunlight by photosynthetic organisms supplies an enormous amount of the energy required to develop and sustain life on the planet, making photosynthesis one of the most important biological phenomenon on Earth. Updating the popular first edition, Photosynthesis Research Protocols, Second Edition presents detailed descriptions of a broad range of general and fundamental methods that are commonly used by plant biochemists, physiologists, and molecular biologists, all of which are contributed by leading researchers in the field. This vital new edition also features coverage of methods related to the most abundant protein on earth, Ribulose-1,5-Bisphosphate Carboxylase/Oxygenase. Written in the highly successful Methods in Molecular Biology™ series format, the chapters of this volume include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and expert tips on troubleshooting and avoiding known pitfalls. Comprehensive and cutting-edge, Photosynthesis Research Protocols, Second Edition serves new photosynthesis researchers as well as experienced scientists seeking to use a new type of preparation or method to study this tremendously influential process.
988			_aSpringer_Protocols_2011
650		7	_2embne _9139651 _aFotosíntesis
776	0	8	_iPrinted edition: _z9781607619246
776	0	8	_iPrinted edition: _z9781607619260
776	0	8	_iPrinted edition: _z9781493956364
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-60761-925-3 _zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
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998			_b06/2023 _dz _eIG _zSI