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008			230606s2007 xxu| o |||| 0|eng d
020			_a9781597452571
024	7		_a10.1007/978-1-59745-257-1 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC _dES-MaUEC
050		4	_aQP84.6 _b2007 EB
245	0	0	_aCircadian Rhythms : _bMethods and Protocols _cedited by Ezio Rosato
250			_a1st edition 2007
264		1	_aTotowa, NJ _bHumana Press _c2007
300			_a1 recurso en línea (XIV, 578 páginas) _b115 ilustraciones
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v362
505	0		_aOverviews -- Light, Photoreceptors, and Circadian Clocks -- Statistical Analysis of Biological Rhythm Data -- Rhythmic Readouts -- Rhythmic Conidiation in Neurospora crassa -- Monitoring and Analyzing Drosophila Circadian Locomotor Activity -- Automated Video Image Analysis of Larval Zebrafish Locomotor Rhythms -- Locomotor Activity in Rodents -- Analysis of Circadian Leaf Movement Rhythms in Arabidopsis thaliana -- Detection of Rhythmic Bioluminescence From Luciferase Reporters in Cyanobacteria -- Analysis of Rhythmic Gene Expression in Adult Drosophila Using the Firefly Luciferase Reporter Gene -- Monitoring Circadian Rhythms in Arabidopsis thaliana Using Luciferase Reporter Genes -- Identification of Clock Genes -- Specialized Techniques for Site-Directed Mutagenesis in Cyanobacteria -- Novel Strategies for the Identification of Clock Genes Neurospora With Insertional Mutagenesis -- Mutagenesis With Drosophila -- Mutagenesis in Arabidopsis -- Yeast Two-Hybrid Screening -- Microarrays -- Microarrays -- Identification of Clock Genes Using Difference Gel Electrophoresis -- Gene Expression: RNA -- Isolation of Total RNA From Neurospora Mycelium -- RNA Extraction From Drosophila Heads -- Extraction of Plant RNA -- RNA Extraction From Mammalian Tissues -- Northern Analysis of Sense and Antisense frequency RNA in Neurospora crassa -- RNase Protection Assay -- Quantitative Polymerase Chain Reaction -- Gene Expression: Proteins -- Protein Extraction, Fractionation, and Purification From Cyanobacteria -- Protein Extraction From Drosophila Heads -- Plant Protein Extraction -- Protein Extraction From Mammalian Tissues -- Western Blotting -- Coimmunoprecipitation Assay -- In Vitro Phosphorylation and Kinase Assays in Neurospora crassa -- In Vitro Systems -- Basic Protocols for Drosophila S2 Cell Line -- Coimmunoprecipitation on Drosophila Cells in Culture -- Basic Protocols for Zebrafish Cell Lines -- Manipulation of Mammalian Cell Lines for Circadian Studies -- Reporter Assays -- Use of Firefly Luciferase Activity Assays to Monitor Circadian Molecular Rhythms In Vivo and In Vitro -- Suprachiasmatic Nucleus Cultures That Maintain Rhythmic Properties In Vitro -- Microscopy Analysis -- RNA In Situ Hybridizations on Drosophila Whole Mounts -- In Situ Hybridization of Suprachiasmatic Nucleus Slices -- Immunohistochemistry in Drosophila -- Immunocytochemistry on Suprachiasmatic Nucleus Slices -- Immunofluorescence Analysis of Circadian Protein Dynamics in Cultured Mammalian Cells.
520			_aThe aim of Circadian Rhythms is to provide a resource that can be adopted by several types of users: those who are new to circadian biology, those who are already active in the field but are interested in learning new techniques and researchers who are considering moving to a new a model system or undertaking comparative studies and would like to consult protocols applied to different organisms before starting the study of new species. This book features a full range of methods that illustrate procedures that have been recently been introduced in circadian studies and by presenting variations to take into account the peculiarities of different model systems.
988			_aSpringer_Protocols_2007
650		7	_2embne _9147045 _aRitmos circadianos
776	0	8	_iPrinted edition: _z9781617375538
776	0	8	_iPrinted edition: _z9781588294173
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-59745-257-1 _zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
942			_2lcc _cLE
998			_b06/2023 _dz _eIG _zSI