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008			230605s2018 xxu| o |||| 0|eng d
020			_a9781493985913
024	7		_a10.1007/978-1-4939-8591-3 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC _dES-MaUEC
050		4	_aTP248.25 .N35 _b2018 EB
245	0	0	_aNanoscale Imaging : _bMethods and Protocols _cedited by Yuri L. Lyubchenko
250			_a1st edition 2018
264		1	_aNew York, NY _bSpringer International Publishing _c2018
300			_a1 recurso en línea (XIX, 600 páginas) _b176 ilustraciones, 170 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v1814
505	0		_aHigh Resolution Atomic Force Microscopy Imaging of Nucleic Acids -- Two Ligand Binding Sites in Serotonin Transporter Revealed by Nanopharmacological Force Sensing -- AFM-Based Single-Molecule Force-Spectroscopy of Proteins -- High Resolution AFM-Based Force Spectroscopy -- Polymer Nanoarray Approach for the Characterization of Biomolecular Interactions -- Measuring Single-Molecule Twist and Torque in Multiplexed Magnetic Tweezers -- AFM-Based Characterization of Electrical Properties of Materials -- Supported Lipid Bilayers for Atomic Force Microscopy Studies -- Quantifying Small Molecule Binding Interactions with DNA Nanostructures -- Optimum Substrates for Imaging Biological Molecules with High-Speed Atomic Force Microscopy -- High Resolution and High-Speed Atomic Force Microscope Imaging -- High-Speed Atomic Force Microscopy of Individual Amyloidogenic Protein Assemblies -- Direct Observation of Dynamic Movement of DNA Molecules in DNA Origami Imaged using High-Speed AFM -- Assembly of Centromere Chromatin for Characterization By High-Speed Time-Lapse Atomic Force Microscopy -- High-Speed Force Spectroscopy for Single Protein Unfolding -- Probing RNA-Protein Interactions with Single-Molecule Pull-Down Assays -- Preparing Frozen-Hydrated Protein-Nucleic Acid Assemblies for High-Resolution Cryo-EM Imaging -- Probing Chromatin Structure with Magnetic Tweezers -- Single Molecule and Ensemble Methods to Probe Initial Stages of RNP Granule Assembly -- Correlative Atomic Force and Single Molecule Fluorescence Microscopy of Nucleoprotein Complexes -- Sensing the Ultrastructure of Bacterial Surfaces and their Molecular Binding Forces using AFM -- Nanoscale Visualization of Bacterial Microcompartments using Atomic Force Microscopy -- Time-Resolved Imaging of Bacterial Surfaces using Atomic Force Microscopy -- Probing Bacterial Adhesion at the Single-Molecule and Single-Cell Levels by AFM-Based Force Spectroscopy -- Fluorescence Correlation Spectroscopy on Genomic DNA in Living Cells -- Three-Dimensional Tracking of Quantum Dot-Conjugated Molecules in Living Cells -- AFM Indentation Analysis of Cells to Study Cell Mechanics and Pericellular Coat -- Imaging of Soft and Biological Samples using AFM Ringing Mode -- Probing Single Virus Binding Sites on Living Mammalian Cells using AFM -- Applications of Atomic Force Microscopy for Adhesion Force Measurements in Mechanotransduction -- Methods for Atomic Force Microscopy of Biological and Living Specimens -- Single Molecule Imaging in Live Embryos using Lattice Light-Sheet Microscopy -- Silver Filler Pre-Embedding to Enhance Resolution and Contrast in Multidimensional SEM: A Nanoscale Imaging Study on Liver Tissue -- Nanoscale Dynamics and Energetics of Proteins and Protein-Nucleic Acid Complexes in Classical Molecular Dynamics Simulations.
520			_aThis volume presents readers with the latest techniques to study nanoimaging and nanoprobing in application to a broad range of biological systems. The chapters in this book are divided into five parts, and cover topics such as imaging and probing of biomacromolecules including high-speed imaging and probing with AFM; probing chromatin structure with magnetic tweezers; and fluorescence correlation spectroscopy on genomic DNA in living cells. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and through, Nanoscale Imaging: Methods and Protocols is a valuable resource for anyone interested in learning more about this developing and expanding field.
988			_aSpringer_Protocols_2018
650		7	_2embne _9158453 _aNanotecnología
650		7	_2embne _9138719 _aMicroscopía
776	0	8	_iPrinted edition: _z9781493985906
776	0	8	_iPrinted edition: _z9781493985920
776	0	8	_iPrinted edition: _z9781493993369
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-4939-8591-3 _zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
942			_2lcc _cLE
998			_b06/2023 _dz _eIG _zSI