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020 _a9781493977208
024 7 _a10.1007/978-1-4939-7720-8
_2doi
040 _aES-MaUEC
_bspa
_cES-MaUEC
245 1 0 _aMouse Retinal Phenotyping
_bMethods and Protocols
_cedited by Naoyuki Tanimoto.
250 _a1st edition 2018
264 1 _aNew York, NY
_bSpringer International Publishing
_c2018
300 _a1 recurso en línea (XI, 334 páginas)
_b75 ilustraciones, 52 ilustraciones a color
336 _atexto
_btxt
_2rdacontent
337 _aelectrónico
_bc
_2rdamedia
338 _arecurso electrónico
_bcr
_2rdacarrier
347 _aarchivo de texto
_bPDF
490 0 _aMethods in Molecular Biology
_x1940-6029
_v1753
505 0 _aMorphological Survey from Neurons to Circuits of the Mouse Retina -- Measuring Retinal Function in the Mouse -- Modeling Retinal Diseases Using Genetic Approaches in Mice -- Cell Culture Analysis of the Phagocytosis of Photoreceptor Outer Segments by Primary Mouse RPE Cells -- Two-photon Microscopy (TPM) and Fluorescence Lifetime Imaging Microscopy (Flim) of Retinal Pigment Epithelium (RPE) of Mice In Vivo -- RPE Visual Cycle and Biochemical Phenotypes of Mutant Mouse Models -- Use of Direct Current Electroretinography for Analysis of Retinal Pigment Epithelium Function in Mouse Models -- Disruption of Rhodopsin Dimerization in Mouse Rod Photoreceptors by Synthetic -- Experimental Approaches for Defining the Role of the Ca2+-Modulated ROS-GC System In Retinal Rods of the Mouse -- Microglia Analysis in Retinal Degeneration Mouse Models -- Determination Of Mitochondrial Oxygen Consumption in the Retina Ex-vivo: Applications for Retinal Disease -- Analysis of Feedback Signaling from Horizontal Cells to Photoreceptors in Mice -- Assessment of the Absolute Excitatory Level of the Retina by Flicker Erg -- Ex Vivo Functional Evaluation of Synaptic Transmission From Rods to Rod Bipolar Cells In Mice -- Functional And Morphological Analysis of Off Bipolar Cells -- Immunohistochemical Phenotyping Of Mouse Amacrine Cell Subtypes -- Phenotyping of Gap-Junctional Coupling in the Mouse Retina -- Ganglion Cell Assessment in Rodents with Retinal Degeneration -- Morphological Identification of Melanopsin Expressing Retinal Ganglion Cell Subtypes in Mice -- Functional Assessment of Melanopsin-driven Light Responses in the Mouse: Multielectrode Array Recordings -- In Vitro Assays for Mouse Müller Cell Phenotyping through Microrna Profiling in the Damaged Retina -- Analysis Of Retinal Vascular Plexi and Interplexus Connections.
520 _aThe aim of this volume is to provide a wide range of techniques and aspects of mouse retinal phenotyping and characterization. Chapters guide readers through morphological and functional properties of the mouse retina and techniques for the analysis of outer to mid-retina and mid- to inner retina. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.   Authoritative and cutting-edge, Mouse Retinal Phenotyping: Methods and Protocols aims to ensure successful results in the further study of this vital field. .
700 1 _aTanimoto, Naoyuki
_eeditor literario
_4edt
_4http://id.loc.gov/vocabulary/relators/edt
776 0 8 _iPrinted edition:
_z9781493977192
776 0 8 _iPrinted edition:
_z9781493977215
776 0 8 _iPrinted edition:
_z9781493992676
856 4 0 _uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-4939-7720-8
_zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
942 _2lcc
_cLE
988 _aSpringer_Protocols_2018
999 _c391891
_d391891