000			04081nam a22003855i 4500
001			391881
003			ES-MaUEC
005			20230102122622.0
007			cr nn 008mamaa
008			160809s2016 gw | s |||| 0|eng d
020			_a9783662491270
024	7		_a10.1007/978-3-662-49127-0 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC
245	1	0	_aHydrocarbon and Lipid Microbiology Protocols _bSynthetic and Systems Biology - Applications _cedited by Terry J. McGenity, Kenneth N. Timmis, Balbina Nogales Fernández.
250			_a1st edition 2016
264		1	_aBerlin, Heidelberg _bSpringer International Publising _c2016
300			_a1 recurso en línea (X, 137 páginas) _b40 ilustraciones, 20 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aSpringer Protocols Handbooks _x1949-2456
505	0		_aIntroduction -- Designing bacteria to produce esters -- Protocols for the production and analysis of isoprenoids in bacteria and yeast -- Synthetic biology of hydrophobic polymer production -- Methods for recombinant rhamnolipid production -- Purification of peptide antimicrobials and thioether stabilized molecules produced in vivo by lantibiotic modification machineries -- Engineering transcription factor based biosensors for the detection of intracellular products -- Oxyfunctionalization of linear alkanes with a biosynthetic, self-sufficient, selective and soluble hydroxylase. .
520			_aThis Volume presents protocols for systems and synthetic biology applications in the field of hydrocarbon and lipid microbiology. It complements another Volume that describes generic protocols for wet experimental and computer-based systems and synthetic biology studies. The protocols in this Volume demonstrate how to employ systems and synthetic biology approaches in the design of microbes for the production of esters, isoprenoids, hydrophobic polymers, rhamnolipid biosurfactant, and peptide antimicrobial and thioether-stabilised molecules. Also presented is a protocol for the engineering of transcription factor-based biosensors for intracellular products, and another for the creation of a synthetic hydroxylase with novel activity for the selective oxyfunctionalisation of linear alkanes. Hydrocarbon and Lipid Microbiology Protocols There are tens of thousands of structurally different hydrocarbons, hydrocarbon derivatives and lipids, and a wide array of these molecules are required for cells to function. The global hydrocarbon cycle, which is largely driven by microorganisms, has a major impact on our environment and climate. Microbes are responsible for cleaning up the environmental pollution caused by the exploitation of hydrocarbon reservoirs and will also be pivotal in reducing our reliance on fossil fuels by providing biofuels, plastics and industrial chemicals. Gaining an understanding of the relevant functions of the wide range of microbes that produce, consume and modify hydrocarbons and related compounds will be key to responding to these challenges. This comprehensive collection of current and emerging protocols will facilitate acquisition of this understanding and exploitation of useful activities of such microbes.
700	1		_aMcGenity, Terry J _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
700	1		_aTimmis, Kenneth N _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
700	1		_aNogales Fernández, Balbina _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
776	0	8	_iPrinted edition: _z9783662491263
776	0	8	_iPrinted edition: _z9783662491287
776	0	8	_iPrinted edition: _z9783662569818
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-3-662-49127-0 _zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
942			_2lcc _cLE
988			_aSpringer_Protocols_2016
999			_c391881 _d391881