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020 _a9781597450003
024 7 _a10.1007/978-1-59745-000-3
_2doi
040 _aES-MaUEC
_bspa
_cES-MaUEC
245 1 0 _aXenopus Protocols
_bCell Biology and Signal Transduction
_cedited by X. Johné Liu.
250 _a1st edition 2006
264 1 _aTotowa, NJ
_bHumana Press
_c2006
300 _a1 recurso en línea (XVI, 489 páginas)
_b
336 _atexto
_btxt
_2rdacontent
337 _aelectrónico
_bc
_2rdamedia
338 _arecurso electrónico
_bcr
_2rdacarrier
347 _aarchivo de texto
_bPDF
490 0 _aMethods in Molecular Biology
_x1940-6029
_v322
505 0 _aResources for Genetic and Genomic Studies of Xenopus -- The Physiology of the Xenopus laevis Ovary -- Oocyte Isolation and Enucleation -- Xenopus tropicalis Oocytes -- Oocyte Expression With Injection of Purified T7 RNA Polymerase -- Visualization of the Cytoskeleton in Xenopus Oocytes and Eggs by Confocal Immunofluorescence Microscopy -- Multiphoton Laser Scanning Microscopy as a Tool for Xenopus Oocyte Research -- Imaging Ca2+ Signals in Xenopus Oocytes -- Chromosomal DNA Replication in a Soluble Cell-Free System Derived From Xenopus Eggs -- Chromatin Assembly of DNA Templates Microinjected Into Xenopus Oocytes -- Pre-mRNA Splicing in the Nuclei of Xenopus Oocytes -- Chromatin Immunoprecipitation for Studying Transcriptional Regulation in Xenopus Oocytes and Tadpoles -- Cytoplasmic mRNA Polyadenylation and Translation Assays -- Xenopus Egg Extracts -- Analysis of Molecular Chaperones Using a Xenopus Oocyte Protein Refolding Assay -- Ubiquitin-Mediated Protein Degradation in Xenopus Egg Extracts -- to Nucleocytoplasmic Transport -- Use of Xenopus laevis Oocyte Nuclei and Nuclear Envelopes in Nucleocytoplasmic Transport Studies -- Nuclear Pore Complex Structure and Plasticity Revealed by Electron and Atomic Force Microscopy -- In Vitro Study of Nuclear Assembly and Nuclear Import Using Xenopus Egg Extracts -- Use of Intact Xenopus Oocytes in Nucleocytoplasmic Transport Studies -- Studying the Mechanosensitivity of Voltage-Gated Channels Using Oocyte Patches -- Oocytes as an Expression System for Studying Receptor/Channel Targets of Drugs and Pesticides -- Microtransplantation of Neurotransmitter Receptors From Cells to Xenopus Oocyte Membranes -- Reconstitution of Golgi Disassembly by Mitotic Xenopus Egg Extract in Semi-Intact MDCK Cells -- Exploring RNA Virus Replication in Xenopus Oocytes -- Study of Apoptosis In Vitro Using the Xenopus Egg Extract Reconstitution System -- Studying Fertilization in Cell-Free Extracts -- Localized Sampling, Electrophoresis, and Biosensor Analysis of Xenopus laevis Cytoplasm for Subcellular Biochemical Assays -- Monitoring Protein Kinase A Activities Using Expressed Substrate in Live Cells -- Using Xenopus Oocyte Extracts to Study Signal Transduction -- Oocyte Extracts for the Study of Meiotic M-M Transition -- Methods for Studying Spindle Assembly and Chromosome Condensation in Xenopus Egg Extracts.
520 _aAlthough Xenopus oocytes are very hardy and robust in terms of experimentation and offer unusually simple and inexpensive opportunities for the delivery of mRNA to the cytoplasm and DNA to the nucleus, they are underutilized as an experimental model. In Xenopus Protocols: Cell Biology and Signal Transduction, hands-on researchers describe standard and cutting-edge techniques for using Xenopus oocytes and oocyte/egg extracts to reconstitute biological and cellular processes. These readily reproducible methods take advantage of the oocyte's impressive protein abundance (for example, 50-70 ng catalytic subunit of PKA per oocyte), its striking protein translation capacity (200-400 ng protein/oocyte per day), and its breathtaking possibilities for the assembly of infectious viral particles by single-cell injection of multiple RNAs. The authors focus on the versatility of frog oocytes and egg extracts in cell biology and signal transduction and cover all the major uses of oocytes/extracts as experimental models. The protocols follow the successful Methods in Molecular Biology™ series format, each offering step-by-step laboratory instructions, an introduction outlining the principles behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls. Comprehensive and highly practical, Xenopus Protocols: Cell Biology and Signal Transduction serves perfectly to instruct novices in the use of Xenopus oocytes/extracts, as well as seasoned investigators interested in developing novel models and uses.
700 1 _aLiu, X. Johné
_eeditor literario
_4edt
_4http://id.loc.gov/vocabulary/relators/edt
776 0 8 _iPrinted edition:
_z9781617375200
776 0 8 _iPrinted edition:
_z9781588293626
856 4 0 _uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-59745-000-3
_zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
942 _2lcc
_cLE
988 _aSpringer_Protocols_2006
999 _c391853
_d391853