000			03593nam a22003855i 4500
001			391845
003			ES-MaUEC
005			20230102122620.0
007			cr nn 008mamaa
008			100301s1999 xxu| s |||| 0|eng d
020			_a9781592596393
024	7		_a10.1385/0896035093 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC
245	1	0	_aIn Vitro Neurochemical Techniques _cedited by Alan A. Boulton, Glen B. Baker, Alan N. Bateson.
250			_a1st edition 1999
264		1	_aTotowa, NJ _bHumana Press _c1999
300			_a1 recurso en línea (XIV, 296 páginas) _b
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aNeuromethods _x1940-6045 _v34
505	0		_aCharacterization of Receptors by Radiolabeled Ligand-Binding Techniques -- Electrophysiological Studies on Receptors In Vitro -- Subtractive Hybridization -- Messenger RNA Differential Display -- Localization of mRNAs in Brain Sections by in situ Hybridization Using Oligonucleotide Probes -- Multiprobe Oligonucleotide Solution Hybridization for the Determination of Relative mRNA Levels -- Competitive RT-PCR to Quantitate Steady-State mRNA Levels -- A Novel PCR-RFLP Detection Method Using an Optimized Set of Restriction Enzymes -- Western Blot Analysis -- The Phosphoinositide Signal Transduction System -- Protein Phosphorylation -- Immunoprecipitation of Receptors.
520			_aIn Vitro Neurochemical Techniques is the third work updating and expanding the best-selling inaugural volume of Humana Press's warmly received Neuromethods series, General Neurochemical Techniques (vol. 1). The key techniques detailed in this new edition encompass the breadth of neurochemical and molecular neurobiology research, ranging from the isolation of neuronal genes and the study of their expression to the analysis of receptor-ligand interactions, to the characterization of the consequences of receptor activation. The methods include electrophysiological techniques to explore the functional properties of receptors present in the membranes of excitable cells, methods to isolate novel genes central to neurobiological processes, and protocols to perform in situ hybridization histochemistry. Other methods cover the measurement of changes in gene expression, the rapid identification of gene polymorphisms, and the identification and characterization of second messenger pathways. The companion volumes, In Vivo Neuromethods and Cell Neurobiology Techniques, cover both in vivo methods and in vitro cell neurobiology approaches. Like the original, all three cutting-edge works will prove exceptionally useful to those basic and clinical neuroscientists who want to expand the range of their current research or develop competence in complementary methods.
700	1		_aBoulton, Alan A _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
700	1		_aBaker, Glen B _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
700	1		_aBateson, Alan N _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
776	0	8	_iPrinted edition: _z9781617370557
776	0	8	_iPrinted edition: _z9780896035096
776	0	8	_iPrinted edition: _z9781489943347
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1385/0896035093 _zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
942			_2lcc _cLE
988			_aSpringer_Protocols_1999
999			_c391845 _d391845