000			04216nam a22003615i 4500
001			391839
003			ES-MaUEC
005			20230102122620.0
007			cr nn 008mamaa
008			100715s2010 xxu| s |||| 0|eng d
020			_a9781603274180
024	7		_a10.1007/978-1-60327-418-0 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC
245	1	0	_aDrug-DNA Interaction Protocols _cedited by Keith R. Fox.
250			_a2nd edition 2010
264		1	_aTotowa, NJ _bHumana Press _c2010
300			_a1 recurso en línea (IX, 311 páginas) _b103 ilustraciones, 1 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v613
505	0		_aQuantitative Analysis of Small Molecule-Nucleic Acid Interactions with a Biosensor Surface and Surface Plasmon Resonance Detection -- Thermal Melting Studies of Ligand DNA Interactions -- Circular and Linear Dichroism of Drug-DNA Systems -- Drug Binding to DNA?RNA Hybrid Structures -- Quantification of Binding Data Using Capillary Electrophoresis -- Determination of Equilibrium Association Constants of Ligand-DNA Complexes by Electrospray Mass Spectrometry -- Detection of Adriamycin-DNA Adducts by Accelerator Mass Spectrometry -- Molecular Modelling Methods to Quantitate Drug-DNA Interactions -- Application of Anomalous Diffraction Methods to the Study of DNA and DNA-Complexes -- DNase I Footprinting -- Methods to Characterize the Effect of DNA-Modifying Compounds on Nucleosomal DNA -- REPSA: Combinatorial Approach for Identifying Preferred Drug-DNA Binding Sequences -- In vitro Transcription Assay for Resolution of Drug-DNA Interactions at Defined DNA Sequences -- In vitro Footprinting of Promoter Regions Within Supercoiled Plasmid DNA -- Topoisomerase I-Mediated DNA Relaxation as a Tool to Study Intercalation of Small Molecules into Supercoiled DNA -- A High-Throughput Assay for DNA Topoisomerases and Other Enzymes, Based on DNA Triplex Formation -- Measurement of DNA Interstrand Crosslinking in Individual Cells Using the Single Cell Gel Electrophoresis (Comet) Assay -- Measurement of DNA Interstrand Crosslinking in Naked DNA Using Gel-Based Methods -- An Evaluation Cascade for G-Quadruplex Telomere Targeting Agents in Human Cancer Cells.
520			_aThe last few years have witnessed the creation of new generations of sequence reading compounds, which have incredible potential for targeting specific DNA sequences. In Drug-DNA Interaction Protocols, Second Edition, expert researchers explore these compounds, revealing how a deeper understanding of the structure of DNA, along with an improved ability to manipulate it, has led to much progress in recent years. Chapters describe a new arsenal of techniques for probing the interactions between drugs and DNA, including various biophysical techniques for quantifying these interactions and for describing them in molecular and atomic detail, and they comprehensively consider both molecular and cellular approaches. Composed in the highly successful Methods in Molecular Biology™ series format, each chapter contains a brief introduction, step-by-step methods, a list of necessary materials, and a Notes section which shares tips on troubleshooting and avoiding known pitfalls. Authoritative and current, Drug-DNA Interaction Protocols, Second Edition, is a critical volume that includes a wide range of chapters, which together provide methods for assessing the strength and mode of binding, the sequence selectivity, and the effect on biological systems.
700	1		_aFox, Keith R _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
776	0	8	_iPrinted edition: _z9781607615194
776	0	8	_iPrinted edition: _z9781617796746
776	0	8	_iPrinted edition: _z9781603274173
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-60327-418-0 _zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
942			_2lcc _cLE
988			_aSpringer_Protocols_2010
999			_c391839 _d391839