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020 _a9781597455275
024 7 _a10.1007/978-1-59745-527-5
_2doi
040 _aES-MaUEC
_bspa
_cES-MaUEC
245 1 0 _aMeiosis
_bVolume 1, Molecular and Genetic Methods
_cedited by Scott Keeney.
250 _a1st edition 2009
264 1 _aTotowa, NJ
_bHumana Press
_c2009
300 _a1 recurso en línea (XII, 372 páginas)
_b63 ilustraciones
336 _atexto
_btxt
_2rdacontent
337 _aelectrónico
_bc
_2rdamedia
338 _arecurso electrónico
_bcr
_2rdacarrier
347 _aarchivo de texto
_bPDF
490 0 _aMethods in Molecular Biology
_x1940-6029
_v557
505 0 _aGenetic Methods for Studying Meiotic Recombination and Chromosome Dynamics -- Interaction of Genetic and Environmental Factors in Saccharomyces cerevisiae Meiosis: The Devil is in the Details -- Optimizing Sporulation Conditions for Different Saccharomyces cerevisiae Strain Backgrounds -- Modulating and Targeting Meiotic Double-Strand Breaks in Saccharomyces cerevisiae -- Methods for Analysis of Crossover Interference in Saccharomyces cerevisiae -- Measurement of Spatial Proximity and Accessibility of Chromosomal Loci in Saccharomyces cerevisiae Using Cre?/loxP Site-Specific Recombination -- Genetic Analysis of Meiotic Recombination in Schizosaccharomyces pombe -- Analysis of Meiotic Recombination in Caenorhabditis elegans -- Visual Markers for Detecting Gene Conversion Directly in the Gametes of Arabidopsis thaliana -- Molecular Analysis of Recombination and Protein-DNA Interactions During Meiosis -- Gel Electrophoresis Assays for Analyzing DNA Double-Strand Breaks in Saccharomyces cerevisiae at Various Spatial Resolutions -- Genome-Wide Mapping of Meiotic DNA Double-Strand Breaks in Saccharomyces cerevisiae -- Detection of Meiotic DNA Breaks in Mouse Testicular Germ Cells -- End-Labeling and Analysis of Spo11-Oligonucleotide Complexes in Saccharomyces cerevisiae -- Detection of SPO11-Oligonucleotide Complexes from Mouse Testes -- Stabilization and Electrophoretic Analysis of Meiotic Recombination Intermediates in Saccharomyces cerevisiae -- Using Schizosaccharomyces pombe Meiosis to Analyze DNA Recombination Intermediates -- Analysis of Chromatin Structure at Meiotic DSB Sites in Yeasts -- Analysis of Protein-DNA Interactions During Meiosis by Quantitative Chromatin Immunoprecipitation (qChIP) -- Genome-Wide High-Resolution Chromatin Immunoprecipitation of Meiotic Chromosomal Proteins in Saccharomyces cerevisiae -- Parallel Detection of Crossovers and Noncrossovers in Mouse Germ Cells -- Analysis of Meiotic Recombination Products from Human Sperm.
520 _aThe unique chromosome dynamics of meiosis have fascinated scientists for well over a century, but in recent years there has been a proliferation of new data concerning the pairing, recombination, and segregation of chromosomes. In Meiosis, expert researchers explore recent advances in three main areas: quantitative genetic methods for analysis of homologous recombination and chromosome pairing, development of direct physical assays for DNA intermediates and products of recombination, and cytological methods for characterizing chromosome behaviors and the patterns by which specific proteins associate with meiotic chromosomes. Meiosis: Volume 1, Molecular and Genetic Methods details methods for culturing and manipulating commonly used model organisms and protocols for detecting and quantifying meiotic recombination events through genetic and physical analysis of DNA-DNA or Protein-DNA interactions. Methods are provided for studies in yeasts, plants, and animals, including mouse and human. Composed in the highly successful Methods in Molecular Biology(tm) series format, each chapter contains a brief introduction, step-by-step methods, a list of necessary materials, and a Notes section which shares tips on troubleshooting and avoiding known pitfalls. Comprehensive and cutting-edge, Meiosis: Volume 1, Molecular and Genetic Methods is an essential guidebook, providing up-to-date and critical new protocols for the study of meiotic chromosome dynamics.
700 1 _aKeeney, Scott
_eeditor literario
_4edt
_4http://id.loc.gov/vocabulary/relators/edt
776 0 8 _iPrinted edition:
_z9781607611011
776 0 8 _iPrinted edition:
_z9781934115664
776 0 8 _iPrinted edition:
_z9781493961573
856 4 0 _uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-59745-527-5
_zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
942 _2lcc
_cLE
988 _aSpringer_Protocols_2009
999 _c391823
_d391823