000 04346nam a22003615i 4500
001 391817
003 ES-MaUEC
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008 130824s2009 xxu| s |||| 0|eng d
020 _a9781597452892
024 7 _a10.1007/978-1-59745-289-2
_2doi
040 _aES-MaUEC
_bspa
_cES-MaUEC
245 1 0 _aPlant Signal Transduction
_bMethods and Protocols
_cedited by Thomas Pfannschmidt.
250 _a1st edition 2009
264 1 _aTotowa, NJ
_bHumana Press
_c2009
300 _a1 recurso en línea (XI, 360 páginas)
_b
336 _atexto
_btxt
_2rdacontent
337 _aelectrónico
_bc
_2rdamedia
338 _arecurso electrónico
_bcr
_2rdacarrier
347 _aarchivo de texto
_bPDF
490 0 _aMethods in Molecular Biology
_x1940-6029
_v479
505 0 _aMultiple Dimensions in Plant Signal Transduction: An Overview -- Phenotyping of Arabidopsis Mutants for Developmental Effects of Gene Deletions -- Phenotyping of Abiotic Responses and Hormone Treatments in Arabidopsis -- Accurate Real-time Reverse Transcription Quantitative PCR -- Probing Spatio-temporal Intracellular Calcium Variations in Plants -- Dynamic Redox Measurements with Redox-Sensitive GFP in Plants by Confocal Laser Scanning Microscopy -- Imaging of Reactive Oxygen Species In vivo -- Identification of Thioredoxin Targeted Proteins Using Thioredoxin Single-Cysteine Mutant-Immobilized Resin -- Determination of in vivo Protein Phosphorylation in Photosynthetic Membranes -- Examining Protein Stability and Its Relevance for Plant Growth and Development -- Probing Circadian Rhythms in Chlamydomonas reinhardtii by Functional Proteomics -- Bimolecular Fluorescence Complementation (BiFC) to Study Protein-protein Interactions in Living Plant Cells -- Fluorescence Cross-Correlation Spectroscopy of Plant Proteins -- The Determination of Protein-protein Interactions by the Mating-based Split-ubiquitin system (mbSUS) -- Functional Complementation of Yeast Mutants to Study Plant Signalling Pathways -- Phosphatase Activities Analyzed by in vivo Expressions -- Chromatin Immunoprecipitation Experiments to Investigate In Vivo Binding of Arabidopsis Transcription Factors to Target Sequences -- Fluorescence-based Electrophoretic Mobility Shift Assay in the Analysis of DNA-binding Proteins -- Analysis of Plant Regulatory DNA sequences by the Yeast-One-Hybrid Assay -- Analysis of Plant Regulatory DNA Sequences by Transient Protoplast Assays and Computer Aided Sequence Evaluation -- RNA-protein Interaction Mediating Post-transcriptional Regulation in the Circadian System.
520 _aDuring recent years, research has greatly expanded our understanding of the sophisticated molecular network of responses which enable plants to develop, survive and propagate under a wide range of conditions. In Plant Signal Transduction: Methods and Protocols, an international panel of experts provide well-established methods vital to analyzing plant signal transduction on the molecular level. Featuring experimental procedures on several of the most popular model organisms, the volume focuses on in planta analyses and the proteins involved in signal transduction in order to aid with the establishment of laboratory techniques or the modification of the protocols for other plants. As part of the highly successful Methods in Molecular Biology™ series, the chapters include brief introductions to the subject, lists of necessary materials, readily reproducible laboratory protocols, and tips on trouble-shooting and avoiding known pitfalls. Comprehensive and cutting-edge, Plant Signal Transduction: Methods and Protocols will benefit plant scientists wishing to improve their experimental approaches and delve further into this exciting and important field of study.
700 1 _aPfannschmidt, Thomas
_eeditor literario
_4edt
_4http://id.loc.gov/vocabulary/relators/edt
776 0 8 _iPrinted edition:
_z9781607610694
776 0 8 _iPrinted edition:
_z9781588299437
776 0 8 _iPrinted edition:
_z9781627038027
856 4 0 _uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-59745-289-2
_zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
942 _2lcc
_cLE
988 _aSpringer_Protocols_2009
999 _c391817
_d391817