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007			cr nn 008mamaa
008			141208s2015 xxu| s |||| 0|eng d
020			_a9781493921751
024	7		_a10.1007/978-1-4939-2175-1 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC
245	1	0	_aPolyadenylation in Plants _bMethods and Protocols _cedited by Arthur G. Hunt, Qingshun Quinn Li.
250			_a1st edition 2015
264		1	_aNew York, NY _bSpringer International Publishing _c2015
300			_a1 recurso en línea (IX, 225 páginas) _b34 ilustraciones, 23 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v1255
505	0		_aComputational Analysis Of Plant Polyadenylation Signals -- Prediction of Plant mRNA Polyadenylation Sites -- Extraction of poly(A) Sites from Large-scale RNA-seq Data -- Poly(A)-tag Deep Sequencing Data Processing To Extract poly(A) Sites -- Analysis of poly(A) Site Choice Using A Java-Based Clustering Algorithm -- RADPRE: a Computational Program for Identification of Differential mRNA Processing Including Alternative Polyadenylation -- Characterization Of Plant Polyadenylation Complexes By Using Tandem Affinity Purification -- In vitro Analysis Of Cleavage And Polyadenylation In Arabidopsis -- Production, Purification, and Assay of Recombinant Proteins for in vitro Biochemical Analyses of the Plant Polyadenylation Complex -- Detection of Disulfide Linkage By Chemical Derivatization And Mass Spectrometry -- Transient Expression Using Agroinfiltration To Study Polyadenylation In Plants -- A 3' RACE Protocol To Confirm Polyadenylation Sites -- Phage Display Library Screening for Identification of Interacting Protein Partners -- Genome-wide Determination Of Poly(A) Site Choice In Plants -- DNA/RNA Hybrid Primer Mediated Poly(A) Tag Library Construction for Illumina Sequencing -- Poly(A) Tag Library Construction from 10 ng Total RNA -- Genome-Wide Analysis Of Distribution of RNA Polymerase II isoforms using ChIP-seq.
520			_aThis volume presents the most recent studies on mRNA polyadenylation in plants. Chapters are divided into three sections covering recent development of the use of bioinformatics tools in the field. Numerous molecular, biochemical, and methods used to characterize polyadenylation sites on a genome-wide scale. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.  Some are specific for plant research, but most can be adopted for research in other organisms. Authoritative and practical, Polyadenylation in Plants: Methods and Protocols provides scientists with a wide range of methods to study mRNA 3'-end formation in plants.
700	1		_aHunt, Arthur G _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
700	1		_aLi, Qingshun Quinn _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
776	0	8	_iPrinted edition: _z9781493921768
776	0	8	_iPrinted edition: _z9781493921744
776	0	8	_iPrinted edition: _z9781493946686
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-4939-2175-1 _zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
942			_2lcc _cLE
988			_aSpringer_Protocols_2015
999			_c391753 _d391753