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001			391743
003			ES-MaUEC
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007			cr nn 008mamaa
008			160720s2016 ja | s |||| 0|eng d
020			_a9784431560302
024	7		_a10.1007/978-4-431-56030-2 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC
245	1	0	_aAdvanced Methods in Structural Biology _cedited by Toshiya Senda, Katsumi Maenaka.
250			_a1st edition 2016
264		1	_aTokyo : _bSpringer Japan : _bImprint: Springer, _c2016
300			_a1 recurso en línea (X, 340 páginas) _b96 ilustraciones, 65 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aSpringer Protocols Handbooks _x1949-2456
505	0		_aPart 1. Protein expression -- 1 Expression in Bacteria and Refolding -- 2 Expression of proteins in insect and mammalian cells -- 3 Application of MultiBac system to large complexes -- 4 Purification using affinity-tag technology -- 5 Cell-Free Protein Production for Structural Biology -- Part 2. Purification and crystallization of membrane proteins -- 6 Overview of membrane protein purification and crystallization -- 7 Cell-free synthesis of membrane proteins -- Part 3. Crystallization and Crystal Engineering -- 8 Screening of cryoprotectants and the multistep soaking method -- 9 Protein modification for crystallization -- Part 4. Interaction analysis -- 10 Analytical ultracentrifugation -- 11 Mass spectrometry -- 12 Frontal gel filtration -- 13 Surface Plasmon Resonance -- Part 5. Advanced methods for structural analyses -- 14 Structural biology with micro-focus beam-lines -- 15 Structural biology and electron microscopy -- 16 Structure determination software for macromolecular X-ray crystallography -- 17 NMR Structural Biology using Paramagnetic Lanthanide Probe.
520			_aThis book provides practical information on a whole set of protein experiments for advanced structural biology, such as X-ray crystallography, NMR, electron microscopy, advanced mass spectroscopy, and surface plasmon resonance, as well as a wide variety of expression systems including eukaryotic and in vitro expression. In the past decade, structural genomics studies have pushed forward the development of automated methods in the field of structural biology, however there is an increasing need for the structural analysis of difficult targets, such as large protein complexes and membrane proteins, which are hard to achieve using conventional automated methods, and require knowledge that goes beyond standard protein chemistry protocols. To address these problems and to help researchers develop novel methods, this volume provides examples of the development of new protein investigation methods and their theoretical background. This book particularly appeals to graduate students, postdoctoral researchers, young investigators wishing to gain a better understanding of the theory behind experiments, and those seeking further advanced, practical structural biology methods.
700	1		_aSenda, Toshiya _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
700	1		_aMaenaka, Katsumi _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
776	0	8	_iPrinted edition: _z9784431560289
776	0	8	_iPrinted edition: _z9784431560296
776	0	8	_iPrinted edition: _z9784431567585
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-4-431-56030-2 _zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
942			_2lcc _cLE
988			_aSpringer_Protocols_2016
999			_c391743 _d391743