000			05125nam a22003615i 4500
001			391732
003			ES-MaUEC
005			20230102122602.0
007			cr nn 008mamaa
008			100427s2010 xxu| s |||| 0|eng d
020			_a9781607617020
024	7		_a10.1007/978-1-60761-702-0 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC
245	1	0	_aPlant Stress Tolerance _bMethods and Protocols _cedited by Ramanjulu Sunkar.
250			_a1st edition 2010
264		1	_aTotowa, NJ _bHumana Press _c2010
300			_a1 recurso en línea (XIV, 386 páginas) _b
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v639
505	0		_aReviews and Overview Chapters -- Dehydration Tolerance in Plants -- Approaches to Identifying Genes for Salinity Tolerance and the Importance of Timescale -- Gene Regulation During Cold Stress Acclimation in Plants -- Redox-Dependent Regulation, Redox Control and Oxidative Damage in Plant Cells Subjected to Abiotic Stress -- Array Platforms and Bioinformatics Tools for the Analysis of Plant Transcriptome in Response to Abiotic Stress -- Metabolic Engineering of Glyoxalase Pathway for Enhancing Stress Tolerance in Plants -- Genome-Wide Approaches for Identification of Stress Regulated Genes, Proteins, and Small RNAs -- Genetic Screens to Identify Plant Stress Genes -- Arabidopsis Tiling Array Analysis to Identify the Stress-Responsive Genes -- Identification of Stress-Responsive Genes in Plants Using Suppression Subtraction Hybridization: Ozone Stress as an Example -- Identification of DNA-Binding Proteins and Protein-Protein Interactions by Yeast One-Hybrid and Yeast Two-Hybrid Screen -- Functional Characterization of Water-Deficit Stress Responsive Genes Using RNAi -- Difference Gel Electrophoresis as a Tool to Discover Stress-Regulated Proteins -- Thiol-Disulfide Redox Proteomics in Plant Research -- Cloning of Stress-Responsive MicroRNAs and other Small RNAs from Plants -- An Array Platform for Identification of Stress-Responsive MicroRNAs in Plants -- Antioxidant Enzymes and Metabolites -- Spectrophotometric Assays for Antioxidant Enzymes in Plants -- Affinity Purification and Determination of Enzymatic Activity of Recombinantly Expressed Aldehyde Dehydrogenases -- Determination and Detection of Reactive Oxygen Species (ROS), Lipid Peroxidation, and Electrolyte Leakage in Plants -- Osmotic Adjustment and Ion Measurements -- Quantification of Water Stress-Induced Osmotic Adjustment and Proline Accumulation for Arabidopsis thaliana Molecular Genetic Studies -- Methods for Determination of Proline in Plants -- A New Method for Accurately Measuring ?1-Pyrroline-5-Carboxylate Synthetase Activity -- Extraction and Analysis of Soluble Carbohydrates -- Measuring Soluble Ion Concentrations (Na+, K+, Cl?) in Salt-Treated Plants.
520			_aA number of abiotic factors such as drought, salinity, extreme temperatures, low or high light intensity, and deficiency or toxic levels of nutrients have huge impacts on crop productivity, and a furthering of our understanding of the molecular, biochemical, and physiological basis of stress tolerance has been widely recognized as critical. In Plant Stress Tolerance: Methods and Protocols, expert researchers cover the most important widely-used techniques, including cutting-edge strategies, in a manner that ensures effective results. Beginning with reviews on dehydration, salinity, and cold tolerance as well as on oxidative stress, the volume then continues with methods involving topics such as describing the identification of stress-regulated genes, proteins, and microRNAs using diverse approaches, measurement of osmotic adjustment, proline levels, enzymes involved in proline metabolism, and sugars as well as determination of ROS levels, lipid peroxidation, ion leakage, and the enzymes involved in ROS detoxification. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective subjects, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Comprehensive and up-to-date, Plant Stress Tolerance: Methods and Protocols provides a wide range of easy-to-follow protocols catering to the needs of plant physiologists, biochemists, and molecular biologists interested in probing this vital area of study.
700	1		_aSunkar, Ramanjulu _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
776	0	8	_iPrinted edition: _z9781607617037
776	0	8	_iPrinted edition: _z9781607617013
776	0	8	_iPrinted edition: _z9781493957415
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-60761-702-0 _zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
942			_2lcc _cLE
988			_aSpringer_Protocols_2010
999			_c391732 _d391732