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020 _a9781592597444
024 7 _a10.1385/1592597440
_2doi
040 _aES-MaUEC
_bspa
_cES-MaUEC
245 1 0 _aGerm Cell Protocols
_bVolume 1: Sperm and Oocyte Analysis
_cedited by Heide Schatten.
250 _a1st edition 2004
264 1 _aTotowa, NJ
_bHumana Press
_c2004
300 _a1 recurso en línea (XIV, 316 páginas)
_b
336 _atexto
_btxt
_2rdacontent
337 _aelectrónico
_bc
_2rdamedia
338 _arecurso electrónico
_bcr
_2rdacarrier
347 _aarchivo de texto
_bPDF
490 0 _aMethods in Molecular Biology
_x1940-6029
_v253
505 0 _aCryopreservation of Semen of the Salmonidae with Special Reference to Large-Scale Fertilization -- Fertilization and Sperm Chemotaxis in Ascidians -- Assays for Vertebrate Sperm Chemotaxis -- Stallion Spermatozoa Viability -- Visualization of Sperm Accessory Structures in the Mammalian Spermatids, Spermatozoa, and Zygotes by Immunofluorescence, Confocal, and Immunoelectron Microscopy -- Localization of Zona Pellucida Receptors on Live Sperm by Fluorophore-Conjugated Solubilized Zona Pellucida Proteins -- Detection of Human Papillomavirus DNA in Sperm Using Polymerase Chain Reaction -- Spermatogonial Stem Cells -- Molecular and Cellular Approaches for Establishment of an In Vitro Spermatogenesis System -- Acrosin Activity Assay for the Evaluation of Mammalian Sperm Acrosome Reaction -- Isolation of Sea Urchin Sperm Plasma Membranes -- Detection of Centrosome Structure in Fertilized and Artificially Activated Sea Urchin Eggs Using Immunofluorescence Microscopy and Isolation of Centrosomes Followed by Structural Characterization with Field Emission Scanning Electron Microscopy -- Microscopic Techniques for Studying Sperm-Oocyte Interaction During Fertilization and Early Embryonic Development -- Immunocytochemical Staining of the Metaphase II Spindle in Mammalian Oocytes -- Spindle Imaging in Living Mammalian Oocytes Using the Liquid-Crystal Polarized Light Microscope and Its Practical Use -- In Vitro Maturation and Fertilization of Pig Oocytes -- In Vitro Maturation and In Vitro Fertilization of Human Oocytes -- In Vitro Maturation and Fertilization of Canine Oocytes -- Detection and Measurement of Membrane-Bound Protein Tyrosine Kinases in the Zebrafish Egg -- Single-Cell Method for Estimation of Protein Tyrosine Kinases in the Zebrafish Egg -- Activity of MAPK/p90rsk During Fertilization in Mice, Rats, and Pigs.
520 _aGerm cell research has produced enormous advances in recent years and more recently has entered into an explosive phase of new discoveries with the introduction of transgenic technologies and nuclear cloning. In Germ Cell Protocols, expert laboratory investigators describe in step-by-step detail a series of powerful techniques for the molecular and genetic analysis of germ cells in a variety of different reproductive systems. Chosen for their proven success in advancing our understanding of germ cells, these methods cover sperm and egg activation, motility, fertilization, nuclear development, nuclear cloning, the molecular characterization of specific events, and the imaging of cell structures. Volume 1: Sperm and Oocyte Analysis focuses on sperm cells, oocyte analysis, oocyte maturation, fertilization, and preparation techniques. Highlights include in vitro maturation and fertilization of human, porcine, and canine oocytes; the cryopreservation of sperm cells; establishment of an in vitro spermatogenesis system; visualization of sperm accessory structures; and motility assays of spermatozoa. Each readily reproducible protocol is described in step-by-step detail and contains an introduction outlining the principle behind the technique, lists of equipment and reagents, and tips on troubleshooting and avoiding known pitfalls. An accompanying second volume, Molecular Embryo Analysis, Live Imaging, Transgenesis, and Cloning, contains methods for molecular egg analysis, live egg imaging, nuclear cloning, oocyte preservation, and nuclear transfer. Comprehensive and cutting-edge, Germ Cell Protocols offers both novice and established researchers a gold-standard collection of hard-to-find methods for high impact research, diverse procedures that are easy-to-follow, well-illustrated, and allow a cross-species transfer of knowledge from lower vertebrates to higher mammalian systems.
700 1 _aSchatten, Heide
_eeditor literario
_4edt
_4http://id.loc.gov/vocabulary/relators/edt
776 0 8 _iPrinted edition:
_z9781617373749
776 0 8 _iPrinted edition:
_z9781588291219
856 4 0 _uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1385/1592597440
_zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
942 _2lcc
_cLE
988 _aSpringer_Protocols_2004
999 _c391729
_d391729