000			03798nam a22003615i 4500
001			391722
003			ES-MaUEC
005			20230102122600.0
007			cr nn 008mamaa
008			130913s2014 xxu| s |||| 0|eng d
020			_a9781627035866
024	7		_a10.1007/978-1-62703-586-6 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC
245	1	0	_aHuman Monoclonal Antibodies _bMethods and Protocols _cedited by Michael Steinitz.
250			_a1st edition 2014
264		1	_aTotowa, NJ _bHumana Press _c2014
300			_a1 recurso en línea (XI, 364 páginas) _b38 ilustraciones, 17 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v1060
505	0		_aHuman Monoclonal Antibodies - The Residual Challenge of Antibody Immunogenicity -- Technical and Ethical Limitations in Making Human Monoclonal Antibodies -- Therapeutic Human Monoclonal Antibodies in Inflammatory Diseases -- Therapeutic Human Monoclonal Antibodies Against Cancer -- Polyclonal and Monoclonal Antibodies in the Clinic -- Production of Human Monoclonal Antibodies by the Epstein- Barr Virus Method -- Humanization and Simultaneous Optimization of Monoclonal Antibody -- Chimeric Antibodies -- Recombinant Genetic Libraries and Human Monoclonal Antibodies -- Production of Stabilized scFv Antibody Fragments in the E. coli Bacterial Cytoplasm -- Construction and Production of an IgG-Like Tetravalent Bispecific Antibody, IgG-Single Chain Fv Fusion -- Construction of Human Antibody Gene Libraries and Selection of Antibodies by Phage Display -- Antigen -Specific Human Monoclonal Antibodies from Transgenic Mice -- Phage Display Technology for Human Monoclonal Antibodies -- Antigen-Specific in vitro Immunization: A Source for Human Monoclonal Antibodies -- Methods for Radiolabelling of Monoclonal Antibodies -- Purification of Human Monoclonal Antibodies and Their Fragments -- Idiotype-Specific IVIG for Therapy of Autoimmune Diseases.
520			_aThe introduction of monoclonal antibodies revolutionized immunology. The development of human monoclonal antibodies was inspired primarily by the enormous clinical benefits promised by these reagents which can be used as anti-inflammatory reagents, anti-tumor reagents and reagents for passive immunization in a variety of pathologies. Human Monoclonal Antibodies: Methods and Protocols presents technical protocols of cellular and molecular methods for the production, purification and application of human monoclonal antibodies, as well as review articles on related topics of human monoclonal and polyclonal antibodies. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.   Authoritative and easily accessible, Human Monoclonal Antibodies: Methods and Protocols seeks to serve both professionals and novices with its well-honed methodologies which will prove invaluable in a clinical setting.
700	1		_aSteinitz, Michael _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
776	0	8	_iPrinted edition: _z9781627035873
776	0	8	_iPrinted edition: _z9781627035859
776	0	8	_iPrinted edition: _z9781493960194
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-62703-586-6 _zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
942			_2lcc _cLE
988			_aSpringer_Protocols_2014
999			_c391722 _d391722