000			03724nam a22003735i 4500
001			391716
003			ES-MaUEC
005			20230102122559.0
007			cr nn 008mamaa
008			100702s2010 xxu| s |||| 0|eng d
020			_a9781603273657
024	7		_a10.1007/978-1-60327-365-7 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC
245	1	0	_aClostridium difficile _bMethods and Protocols _cedited by Peter Mullany, Adam P. Roberts.
250			_a1st edition 2010
264		1	_aTotowa, NJ _bHumana Press _c2010
300			_a1 recurso en línea (X, 241 páginas) _b
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v646
505	0		_ato Clostridium difficile and the Disease It Causes -- Clostridium difficile: No Longer an Enigmatic Pathogen? -- Clostridium difficile and the Disease It Causes -- Isolation and Culture Techniques -- Clostridium difficile Isolation and Culture Techniques -- Methods for Typing and Epidemiological Studies -- Molecular Typing Methods for Clostridium difficile: Pulsed-Field Gel Electrophoresis and PCR Ribotyping -- Clostridium difficile Toxinotyping -- Multilocus Sequence Typing for Clostridium difficile -- Biochemistry of the Organism -- Molecular Methods to Study Transcriptional Regulation of Clostridium difficile Toxin Genes -- Dissecting the Cell Surface -- Human Intestinal Epithelial Response(s) to Clostridium difficile -- Genomics -- Comparative Genome Analysis of Clostridium difficile Using DNA Microarrays -- Development of Systems for Genetic Analysis of the Organism -- ClosTron-Targeted Mutagenesis -- Methods for Gene Cloning and Targeted Mutagenesis -- Transposon Mutagenesis in Clostridium difficile -- Animal Models of Disease -- Refinement of the Hamster Model of Clostridium difficile Disease -- Methods for Working with the Mouse Model.
520			_aClostridium difficile, a major nosocomial pathogen shown to be a primary cause of antibiotic-associated disease, has emerged as a highly transmissible and frequently antibiotic-resistant organism, causing a considerable burden on health care systems worldwide. In Clostridium difficile: Methods and Protocols, expert researchers bring together the most recently developed methods for studying the organism, including techniques involving isolation, molecular typing, genomics, genetic manipulation, and the use of animal models. Written in the highly successful Methods in Molecular Biology™ series format, chapters include brief introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes highlighting tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Clostridium difficile: Methods and Protocols serves as an ideal guide for scientists now in a position to gain an in-depth understanding of how this organism is transmitted and how it causes disease.
700	1		_aMullany, Peter _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
700	1		_aRoberts, Adam P _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
776	0	8	_iPrinted edition: _z9781607616948
776	0	8	_iPrinted edition: _z9781603273640
776	0	8	_iPrinted edition: _z9781493957019
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-60327-365-7 _zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
942			_2lcc _cLE
988			_aSpringer_Protocols_2010
999			_c391716 _d391716