000			04858nam a2200397 i 4500
999			_c391618 _d391618
001			391618
003			ES-MaUEC
005			20230714080135.0
006			a|||| o|||| 00| 0
007			cr nn 008mamaa
008			180512s2018 xxua o |||| 0|eng d
020			_a9781493977956
024	7		_a10.1007/978-1-4939-7795-6 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC _dES-MaUEC
050		4	_aTA164 _b2018 EB
245	0	0	_aSynthetic Biology : _bMethods and Protocols _cedited by Jeffrey Carl Braman
250			_a1st edition 2018
264		1	_aNew York, NY _bSpringer International Publishing _c2018
300			_a1 recurso en línea (XV, 510 páginas) _b109 ilustraciones, 82 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v1772
505	0		_aRe-Factoring Glycolytic Genes for Targeted Engineering of Catabolism in Gram-Negative Bacteria -- Negative Regulation Gene Circuits for Efflux Pump Control -- Methods for Generating Microbial Co-Cultures that Grow in the Absence of Fixed Carbon or Nitrogen -- Synthetic Biology with an All E. coli TXTL System: Quantitative Characterization of Regulatory Elements and Gene Circuits -- Transformation of an Exotic Yeast Species into a Platform Organism: A Case Study for Engineering Glycolipid Production in the Yeast Starmerella bombicola -- Sortase A-Assisted Metabolic Enzyme Ligation in Escherichia coli for Enhancing Metabolic Flux -- Construction of CRISPR Libraries for Functional Screening -- Targeted Genome Editing Using DNA-Free RNA-Guided Cas9 Ribonucleoprotein for CHO Cell Engineering -- Dynamic Management of Codon Compression for Saturation Mutagenesis -- Designed Transcriptional Regulation in Mammalian Cells Based on TALE- and CRISPR/dCas9 -- Combinatorial Evolution of DNA with RECODE -- Genome Editing in Penicillium chrysogenum Using Cas9 Ribonucleoprotein Particles -- Targeted Gene Knockout Using Nuclease-Assisted Vector Integration: Hemi- and Homozygous Deletion of JAG1 -- Programming Biomaterial Interactions Using Engineered Living Cells -- Rapid Control of Genome Editing in Human Cells by Chemical-Inducible CRISPR-Cas Systems -- Production of a Human Cell Line with a Plant Chromosome -- Bacterial Genome Editing with CRISPR-Cas9: Taking Clostridium beijerinckii as an Example -- CRISPR-Cas9-Mediated Genome Editing and Transcriptional Control in Yarrowia lipolytica -- Construction and Integration of a Synthetic MicroRNA Cluster for Multiplex RNA Interference in Mammalian Cells -- Bio-Algorithmic Workflows for Standardized Synthetic Biology Constructs -- Engineering Genomes with Genotype Specification Language -- Automated Visualization of Genetic Designs Using DNAplotlib -- DNA-Based Analog Computing -- DNA Assembly with the DATEL Method -- EcoFlex: A Multifunctional MoClo Kit for E. coli Synthetic Biology -- Designing and Assembling Plasmids for Construction of Escherichia coli Biosensor for Vibrio cholerae Detection -- A Rapid Combinatorial Approach to Assembling Synthetic Prokaryotic and Eukaryotic Protein Expression Vectors -- Standardized Cloning and Curing of Plasmids -- STAR: A Simple TAL Effector Assembly Reaction Using Isothermal Assembly.
520			_aThis detailed book explores a few of the plethora of techniques and applications associated with the unique branch of science known as synthetic biology. Chemists, biologists, and engineers engaged in this multidisciplinary field of study will be guided in the creation and regulation of gene circuits, manipulation of biochemical pathways, genome editing and modification, creating genome language and computing, as well as molecular assembly. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible protocols, plus troubleshooting tips and explanations of how to avoid pitfalls, all written by experts who have published their work in peer-reviewed journals. Authoritative and practical, Synthetic Biology: Methods and Protocols provides key guidance and ideas for conducting your own synthetic biology projects.
988			_aSpringer_Protocols_2018
650		7	_2embne _9139084 _aBioingeniería
776	0	8	_iPrinted edition: _z9781493977949
776	0	8	_iPrinted edition: _z9781493977963
776	0	8	_iPrinted edition: _z9781493992874
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-4939-7795-6 _zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
942			_2lcc _cLE
998			_b07/2023 _dz _eb _zSI