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008			180507s2018 xxua o |||| 0|eng d
020			_a9781493978472
024	7		_a10.1007/978-1-4939-7847-2 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC _dES-MaUEC
050		4	_aRM301.25 _b2018 EB
245	0	0	_aPhenotypic Screening : _bMethods and Protocols _cedited by Bridget Wagner
250			_a1st edition 2018
264		1	_aNew York, NY _bSpringer International Publishing _c2018
300			_a1 recurso en línea (XII, 280 páginas) _b52 ilustraciones, 36 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v1787
505	0		_aNutrient Stress Small-Molecule Screening Platform for Escherichia coli -- Validation and Development of an Escherichia coli Riboflavin Pathway Phenotypic Screen Hit as a Small-molecule Ligand of the Flavin Mononucleotide Riboswitch -- Phenotypic Screening of Small Molecules with Anti-malarial Activity for Three Different Parasitic Life Stages -- Phenotypic Screening for Inhibitors of a Mutant Thrombopoietin Receptor -- A cell-based Assay for Mitotic Spindle Orientation -- Isolation of Skeletal Muscle Stem Cells for Phenotypic Screens for Modulators of Proliferation -- Cell-based Methods to Identify Inducers of Human Pancreatic Beta-cell Proliferation -- High-content Imaging Phenotypic Screen for Neurogenesis Using Primary Neural Progenitor Cells -- Discovery of Modulators of Adipocyte Physiology Using Fully Functionalized Fragments -- The Ancient Genetic Networks of Obesity: Whole-animal Automated Screening for Conserved Fat Regulators -- Modulation of Threat Response in Larval Zebrafish -- A PLA-iRoCS Pipeline for the Localization of Protein-Protein Interactions in situ -- High-Dimensional Profiling: The Theta Comparative Cell Scoring Method -- Accessing the Open PHACTS Discovery Platform with workflow tools -- Quantitative Prioritization of Tool Compounds for Phenotypic Screening -- A Quantitative Systems Pharmacology Approach to Infer Pathways Involved in Complex Disease Phenotypes -- Phenotype-based High-content Screening using Fluorescent Chemical Bioprobes: Lipid Droplets and Glucose Uptake Quantification in Live Cells -- Developing High-throughput Electrophysiological Assays for Phenotypic Screening -- Testing Susceptibility of Patient Derived Organoid Cultures to Therapies: Pharmacotyping -- Techniques to Enable 1536-well Phenotypic Screening. .
520			_aThis volume provides a better understanding of the advancements in phenotypic readouts and improved disease models that generate novel biological insights and recapitulate clinically relevant biology. Chapters focus on phenotypic screening, the use of human cell models, microscopic approaches, assays to measure fat accumulation in C. elegans, the threat response in zebrafish, and protein-protein interactions in plant growth and development. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Phenotypic Screening: Methods and Protocols aims to make phenotypic screening approaches more accessible to a wide array of researchers throughout the academic and biotech communities.
988			_aSpringer_Protocols_2018
650		7	_2embne _9183058 _aFarmacología _xInvestigación
776	0	8	_iPrinted edition: _z9781493978465
776	0	8	_iPrinted edition: _z9781493978489
776	0	8	_iPrinted edition: _z9781493993031
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-4939-7847-2 _zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
942			_2lcc _cLE
998			_b07/2023 _dz _eb _zSI