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008			171202s2018 xxua o |||| 0|eng d
020			_a9781493974139
024	7		_a10.1007/978-1-4939-7413-9 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC _dES-MaUEC
050		4	_aQP751 _b2018 EB
245	0	0	_aSphingosine-1-Phosphate : _bMethods and Protocols _cedited by Alice Pébay, Kursad Turksen
250			_a2nd edition 2018
264		1	_aNew York, NY _bSpringer International Publishing _c2018
300			_a1 recurso en línea (X, 182 páginas) _b26 ilustraciones
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v1697
505	0		_aMeasuring Sphingosine-1-Phosphate/Protein Interactions with the Kinetic Exclusion Assay -- An Improved Isoform-Selective Assay for Sphingosine Kinase 1 Activity -- Methods for Analyzing Sphingosine-1-Phosphate Signaling in Human and Mouse Primary Mast Cells -- Measurement of Lysophosphatidic Acid and Sphingosine-1-Phosphate by Liquid Chromatography-Coupled Electrospray Ionization Tandem Mass Spectrometry -- Immunohistochemical Detection of Sphingosine-1-Phosphate and Sphingosine Kinase-1 in Human Tissue Samples and Cell Lines -- A Clean-Up Method for Mass Spectrometric Analysis of Sphingosine-1-Phosphate in Blood and Solid Tissues Using a Phosphate Capture Molecule -- A Rapid Fluorescence Assay for Measuring Sphingosine-1-Phosphate Transporter Activity in Erythrocytes -- Analysis of S1P Receptor Expression by Uterine Immune Cells Using Standardized Multi-Parametric Flow Cytometry -- S1P Synergizes with Wall Shear Stress and Other Angiogenic Factors to Induce Endothelial Cell Sprouting Responses -- In Vitro Methods to Study the Modulation of Migration and Invasion by Sphingosine-1-Phosphate -- Maintenance of Human Embryonic Stem Cells by Sphingosine-1-Phosphate and Platelet-Derived Growth Factor -- Sphingosine-1-Phosphate (S1P) Signaling in Neural Progenitors -- Ceramide and S1P Signaling in Embryonic Stem Cell Differentiation -- 3D Stacked Construct: A Novel Substitute for Corneal Tissue Engineering.
520			_aThis fully updated edition explores methods involving sphingosine-1-phosphate (S1P), a bioactive lysophospholipid which has become the focus of much research interest as it has widespread developmental and physio-pathological actions, controlling events within the nervous, reproductive, gastrointestinal, vascular, respiratory, and immune systems, in addition to having a prominent role in cancer, early mammalian embryogenesis, and stem cells. Here, worldwide experts in the S1P field describe in-depth techniques in an array of cell types and with various physiological applications, showcasing the important effects of S1P in development and in physiopathology. As a volume in the Methods in Molecular Biology series, chapters contain introductions to their respective topics, lists of the relevant materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips for troubleshooting and avoiding known pitfalls. Authoritative and timely, Sphingosine-1-Phosphate: Methods and Protocols, Second Edition is another key resource for scientists working in this fast-moving and dynamic field.
988			_aSpringer_Protocols_2018
650		7	_2embne _9142933 _aLípidos
776	0	8	_iPrinted edition: _z9781493974122
776	0	8	_iPrinted edition: _z9781493974146
776	0	8	_iPrinted edition: _z9781493984787
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-4939-7413-9 _zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
942			_2lcc _cLE
998			_b07/2023 _dz _eb _zSI