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008			210604s2021 xxua o |||| 0|eng d
020			_a9781071614990
024	7		_a10.1007/978-1-0716-1499-0 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC _dES-MaUEC
050		4	_aQP623 _b2021 EB
245	0	0	_aRNA Scaffolds : _bMethods and Protocols _cedited by Luc Ponchon
250			_a2nd edition 2021
264		1	_aNew York, NY _bSpringer International Publising _c2021
300			_a1 recurso en línea (XIII, 288 páginas) _b61 ilustraciones, 41 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v2323
505	0		_aPredicting RNA Scaffolds with a Hybrid Method of Vfold3D and VfoldLA -- RNA Footprinting Using Small Chemical Reagents -- Improving RNA Crystal Diffraction Quality by Post-Crystallization Treatment -- Using tRNA Scaffold to Assist RNA Crystallization -- RNA Modeling with the Computational Energy Landscape Framework -- Co-Expression and Co-Purification of RNA-Protein Complexes in Escherichia coli -- In Vivo Production of Small Recombinant RNAs Embedded in 5S rRNA-Derived Protective Scaffold -- Production of Circular Recombinant RNA in Escherichia coli Using Viroid Scaffolds -- Identification of RNA-Binding Proteins Associated to RNA Structural Elements -- Live Cell Imaging Using Riboswitch-Spinach tRNA Fusions as Metabolite-Sensing Fluorescent Biosensors -- Rational Design of Allosteric Fluorogenic RNA Sensors for Cellular Imaging -- Riboswitch-Mediated Detection of Metabolite Fluctuations During Live Cell Imaging of Bacteria -- FRET-Analysis of RNA-Protein-Interactions using Spinach-Aptamers -- Engineering Aptazyme Switches for Conditional Gene Expression in Mammalian Cells Utilizing an In Vivo Screening Approach -- Aptazyme-Based Riboswitches and Logic Gates in Mammalian Cells -- Folding RNA-Protein Complex into Designed Nanostructures -- An Effective Method for Specific Gene Silencing in Escherichia coli Using Artificial Small RNA -- Expression and Purification of tRNA/pre-miRNA-Based Recombinant Noncoding RNAs -- Synthetic Biology Medicine and Bacteria-Based Cancer Therapeutics.
520			_aThis second edition volume expands on the previous edition with discussions of recently developed techniques that use RNA scaffolds as molecular tools. These latest methods are used in the fields of molecular biology, cellular biology, nanotechnology, and structural biology. The chapters in this book cover topics such as RNA footprinting using small chemical reagents; using tRNA scaffolds to assist RNA crystallization; identification of RNA-binding proteins associated to RNA structural elements; and synthetic biology medicine and bacteria-based cancer therapeutics. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and comprehensive, RNA Scaffolds: Methods and Protocols, Second Edition is a valuable resource that explores the fascinating uses of RNA in these different areas, and will inspire experts and novice researchers to further study this developing field. .
988			_aSpringer_Protocols_2021
650		7	_2embne _9144940 _aARN
776	0	8	_iPrinted edition: _z9781071614983
776	0	8	_iPrinted edition: _z9781071615003
776	0	8	_iPrinted edition: _z9781071615010
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-0716-1499-0 _zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
942			_2lcc _cLE
998			_b07/2023 _dz _eb _zSI