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008			210524s2021 xxua o |||| 0|eng d
020			_a9781071614020
024	7		_a10.1007/978-1-0716-1402-0 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC _dES-MaUEC
050		4	_aQH224 _b2021 EB
245	0	0	_aConfocal Microscopy : _bMethods and Protocols _cedited by Joseph Brzostowski, Haewon Sohn
250			_a1st edition 2021
264		1	_aNew York, NY _bSpringer International Publising _c2021
300			_a1 recurso en línea (XI, 363 páginas) _b88 ilustraciones, 73 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v2304
505	0		_aAdvances in Confocal Microscopy and Selected Applications -- Choosing Fluorescent Probes and Labeling Systems -- General Considerations for Acquiring a Three-color Image by Laser Scanning Confocal Microscopy -- Microfabricated devices for confocal microscopy on biological samples -- Zeiss Airyscan: Optimizing Usage for Fast, Gentle, Super-resolution Imaging -- High-resolution multicolor imaging of mitochondria in lymphocytes -- Protein-Retention Expansion Microscopy (ExM): Scalable and Convenient Super-Resolution Microscopy -- Analysis of B Cell Receptor-Mediated Antigen Extraction by B lymphocytes from Plasma Membrane Sheets Using Confocal Microscopy -- Analysis of Intracellular Vesicles in B Lymphocytes: Antigen Traffic in the Spotlight -- Visualizing Key Signaling Components of Macropinocytosis and Phagocytosis Using Confocal Microscopy in the Model Organism Dictyostelium discoideum -- Imaging GPCR-mediated Signal Events Leading to Chemotaxis and Phagocytosis -- High-throughput Imaging of Arrays of Fluorescently Tagged Yeast Mutant Strains -- Visualizing the Dynamics of T Cell-Dendritic Cell Interactions in Intact Lymph Nodes By Multiphoton Confocal Microscopy -- Studying Neuronal Biology Using Spinning Disc Confocal Microscopy -- Method for Acute Intravital Imaging of the Large Intestine in Live Mice -- Fluorescence Lifetime Imaging as a Non-invasive Tool to Study Plasmodium Falciparum Metabolism -- Developing Analysis Protocols for Monitoring Intracellular Oxygenation using Fluorescence Lifetime Imaging of Myoglobin-mCherry -- FLIM Imaging for Metabolic Studies in Live Cells -- A Step by Step Guide to Instant Structured Illumination Microscopy (ISIM).
520			_aThis volume provides a wide range of imaging protocols that can be tailored to specific organisms or cell-types. Chapters guide readers through fixed-cell, live-cell, phenotype screening, super-resolution, intravital imaging techniques, and fluorescence life-time imaging microscopy (FLIM). Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Confocal Microscopy: Methods and Protocols aims to ensure successful results in the further study of this vital field.
988			_aSpringer_Protocols_2021
650		7	_2embne _9163357 _aMicroscopía confocal
776	0	8	_iPrinted edition: _z9781071614013
776	0	8	_iPrinted edition: _z9781071614037
776	0	8	_iPrinted edition: _z9781071614044
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-0716-1402-0 _zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
942			_2lcc _cLE
998			_b07/2023 _dz _eb _zSI