000 04375nam a2200409 i 4500
999 _c391532
_d391532
001 391532
003 ES-MaUEC
005 20230704134136.0
006 a|||| o|||| 00| 0
007 cr nn 008mamaa
008 180430s2018 xxua o |||| 0|eng d
020 _a9781493978410
024 7 _a10.1007/978-1-4939-7841-0
_2doi
040 _aES-MaUEC
_bspa
_cES-MaUEC
_dES-MaUEC
050 4 _aQR186.5
_b2018 EB
245 0 0 _aEpitope Mapping Protocols
_cedited by Johan Rockberg, Johan Nilvebrant
250 _a3rd edition 2018
264 1 _aNew York, NY
_bSpringer International Publishing
_c2018
300 _a1 recurso en línea (XI, 284 páginas)
_b49 ilustraciones, 38 ilustraciones a color
336 _atexto
_btxt
_2rdacontent
337 _aelectrónico
_bc
_2rdamedia
338 _arecurso electrónico
_bcr
_2rdacarrier
347 _aarchivo de texto
_bPDF
490 0 _aMethods in Molecular Biology
_x1940-6029
_v1785
505 0 _aAn Introduction to Epitope Mapping -- Epitope Mapping of Antibody-Antigen Interactions with X-Ray Crystallography -- Mapping Antibody Epitopes by Solution NMR Spectroscopy: Practical Considerations -- Label-Free Cell-Based Assay for Characterization of Biomolecules and Receptors -- Probing Antibody Binding Sites on G Protein-Coupled Receptors Using Genetically-Encoded Photo-Activatable Cross-Linkers -- Rapid Mapping of Protein Binding Sites and Conformational Epitopes by Coupling Yeast Surface Display to Chemical Labeling and Deep Sequencing -- Epitope Mapping Using Yeast Display and Next Generation Sequencing -- B-Cell Epitope Mapping Using a Library of Overlapping Synthetic Peptides in an Enzyme-Linked Immunosorbent Assay -- Array-In-Well Epitope Mapping of Phage-Displayed Antibodies -- Epitope Mapping of Antibodies Using Bacterial Cell Surface Display of Gene Fragment Libraries -- Discontinuous Epitope Mapping of Antibodies Using Bacterial Cell Surface Display of Folded Domains -- Kinetic Analysis and Epitope Binning Using Surface Plasmon Resonance -- Epitope Binning of Human Monoclonal Antibodies in Classical Sandwich and In-Tandem Orientation Using the Octet System Based on Biolayer Interferometry -- Antibody Specificity Profiling Using Protein Microarrays -- High-Density Antigen Microarrays for Assessment of Antibody Selectivity and Off-Target Binding -- Multiplexed Antigen Bead Arrays for Assessment of Antibody Selectivity and Epitope Mapping -- Antibody Biomarker Discovery Via In Vitro Evolution of Peptides -- Bioinformatics Tools for the Prediction of T Cell Epitopes.
520 _aThis third edition volume expands on the previous editions with more detailed research on the characterization of antibody antigen interactions between different users with different requirements. The chapters in this book are divided into four parts: Part One looks at the entire native antigen and covers traditional structural biology techniques such as nuclear magnetic resonance and x-ray crystallography. Part Two talks about protein fragments derived from antigens, and discusses binding regions within antigen sequence using bacterial surface display and ELISA, for example. Part Three describes the use of surface plasmon resonance spectroscopy and biolayer interferometry, and Part Four highlights methods used to identify new antigens and assess antibody cross-reactivity. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Thorough and cutting-edge, Epitope Mapping Protocols, Third Edition is a valuable resource for anyone interested in furthering their research in this expanding field.
988 _aSpringer_Protocols_2018
650 7 _2embne
_9138531
_aAntígenos
650 7 _2embne
_9138330
_aInmunología
776 0 8 _iPrinted edition:
_z9781493978397
776 0 8 _iPrinted edition:
_z9781493978403
776 0 8 _iPrinted edition:
_z9781493993017
856 4 0 _uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-4939-7841-0
_zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
942 _2lcc
_cLE
998 _b07/2023
_dz
_eb
_zSI