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008			171124s2018 xxua o |||| 0|eng d
020			_a9781493975198
024	7		_a10.1007/978-1-4939-7519-8 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC _dES-MaUEC
050		4	_aQR185.2 _b2018 EB
245	0	0	_aInnate Immune Activation : _bMethods and Protocols _cedited by Dominic De Nardo, Christine M De Nardo
250			_a1st edition 2018
264		1	_aNew York, NY _bSpringer International Publishing _c2018
300			_a1 recurso en línea (XIII, 251 páginas) _b54 ilustraciones, 40 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v1714
505	0		_aEmerging concepts in innate immunity -- Bioinformatic Assessment of macrophage activation by the innate immune system -- Generation of Genetic Knockouts in myeloid cell lines using a lentiviral CRISPR/Cas9 system -- Modeling primary human monocytes with the trans-differentiation cell line BLaER1 -- Measurement of NF-κB activation in TLR-activated macrophages -- Biochemical isolation of the myddosome from murine macrophages -- Generation of innate immune reporter cell lines using retroviral transduction -- Myddosome Formation by Luminescence-based Mammalian Interactome Mapping (LUMIER) -- Inflammatory caspases: Activation and cleavage of Gasdermin-D in vitro and during Pyroptosis -- Methods for detecting ASC and ASC speck formation -- Measuring innate immune responses to bacterial viability -- Methods to study cell swelling-induced inflammasome activation -- Detecting release of bacterial dsDNA into the host cytosol using fluorescence microscopy -- Quantitative proteomics of secreted proteins -- Simultaneous detection of cellular viability and interleukin-1β secretion from single cells by ELISpot -- Detection and quantification of MAV aggregation via confocal imaging. .
520			_aThis volume presents standard approaches and the most recent technical advances used to study innate immune activation. Chapters detail the assessment of macrophage activation, measuring innate immune responses to bacterial viability, quantification of secreted proteins, reporter systems, protocols examining specific innate immune activation by TLRs, RLRs, cGAS, and inflammasomes. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Innate Immune Activation: Methods and Protocols aims to be a useful and informative reference tool for further study into this vital field.
988			_aSpringer_Protocols_2018
650		7	_2embne _9145491 _aInmunidad
776	0	8	_iPrinted edition: _z9781493975181
776	0	8	_iPrinted edition: _z9781493975204
776	0	8	_iPrinted edition: _z9781493985081
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-4939-7519-8 _zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
942			_2lcc _cLE
998			_b06/2023 _dz _eb _zSI