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008			100301s2003 xxua o |||| 0|eng d
020			_a9781592593941
024	7		_a10.1385/1592593941 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC _dES-MaUEC
050		4	_aRB112.5 _b2003 EB
245	0	0	_aProtein Misfolding and Disease _cedited by Peter Bross, Niels Gregersen
250			_a1st edition 2003
264		1	_aTotowa, NJ _bHumana Press _c2003
300			_a1 recurso en línea (XV, 318 páginas)
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v232
505	0		_aGeneral Concepts and Models -- Protein Misfolding, Aggregation, and Degradation in Disease -- Basic Introduction to In Vivo Protein Folding and Its Defects -- Cystic Fibrosis -- ?1-Antitrypsin Deficiency -- Parkinson's Disease -- Aberrant Protein Folding as the Molecular Basis of Cancer -- Yeast as a Model System to Investigate Protein Conformational Diseases -- General Methods -- Expression of Recombinant Proteins -- Expression in E. coli Systems -- Protein Expression in Yeasts -- Site-Directed Mutagenesis -- Pulse-Chase Labeling Techniques for the Analysis of Protein Maturation and Degradation -- Techniques in Conformational Disease Research -- Detection of Aggregates and Protein Inclusions by Staining of Tissues -- Study of Mutant Proteins With Folding Defects in Cultured Patient Cells -- E. coli Expression System for Identifying Folding Mutations of Human Adenosine Deaminase -- Characterization of Overexpressed Mutant Proteins in Mammalian Cells -- Investigation of Unfolded-Protein Response in Cells Expressing Familial Alzheimer's Disease-Linked Presenilin Variants -- Huntingtin Fragments Form Aggresome-Like Inclusion Bodies in Mammalian Cells -- Application of Chemical Chaperones to the Rescue of Folding Defects -- Analysis of Defective Subunit Interactions Using the Two-Hybrid System -- Investigation of Folding and Degradation of In Vitro Synthesized Mutant Proteins in the Cytosol -- Investigation of Folding and Degradation of In Vitro Synthesized Mutant Proteins in Microsomes -- Investigation of Folding and Degradation of In Vitro Synthesized Mutant Proteins in Mitochondria -- Investigation of Folding and Degradation of Mutant Proteins Synthesized in Semipermeabilized Cells.
520			_aIt has now become clear that a large number of diseases with very different pathologies share a common framework of protein misfolding, accompanied by degradation and/or aggregation of the misfolded proteins. In Protein Misfolding and Disease: Principles and Protocols, notable experts in conformational disease review the latest thinking about the molecular processes underlying these diseases and describe cutting-edge biochemical, genomic, cellular, and chemical laboratory techniques for studying their genesis and pathologies. The authors apply their carefully refined methods to a variety of metabolic and neurodegenerative disorders, as well as to the aging process. The techniques presented are broadly applicable in many diverse disease contexts and may be used in both diagnosis and detailed research on new treatment strategies. Each tried and proven protocol includes insightful background notes, lists of required equipment and reagents, step-by-step instructions, and tips on troubleshooting and on how to avoid known pitfalls. Comprehensive and cutting-edge, Protein Misfolding and Disease: Principles and Protocols offers both novice and experienced researchers a solid theoretical grounding in conformational disease and a remarkable set of analytical methodologies for uncovering its characteristics and generating new therapeutic approaches.
988			_aSpringer_Protocols_2003
650		7	_2embne _9149316 _aBioquímica clínica
776	0	8	_iPrinted edition: _z9781617373329
776	0	8	_iPrinted edition: _z9781588290656
776	0	8	_iPrinted edition: _z9781489938435
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1385/1592593941 _zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
942			_2lcc _cLE
998			_b07/2023 _dz _eb _zSI