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008			100301s2001 xxu| o |||| 0|eng d
020			_a9781592592074
024	7		_a10.1385/1592592074 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC _dES-MaUEC
050		4	_aQP356.25 _b2001 EB
245	0	0	_aProtocols for Neural Cell Culture _cedited by Sergey Fedoroff, Arleen Richardson
250			_a3rd edition 2001
264		1	_aTotowa, NJ _bHumana Press _c2001
300			_a1 recurso en línea (XXII, 262 páginas)
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aSpringer Protocols Handbooks _x1949-2456
505	0		_aIn Vitro Assays for Axonal Growth and Targeting -- Interface Organotypic Hippocampal Slice Cultures -- Slice Cultures for Study of Microglia -- Microexplant Cultures of the Cerebellum -- Construction and Use of Compartmented Cultures for Studies of Cell Biology of Neurons -- Chick Spinal Somatic Motoneurons in Culture -- Primary Cultures of Sympathetic Ganglia -- Primary Cell Cultures for the Study of Myelination -- Preparation of Astrocyte, Oligodendrocyte, and Microglia Cultures from Primary Rat Cerebral Cultures -- Culture of Glial Cells from Human Brain Biopsies -- Cultures of Astroglia and Microglia from Primary Cultures of Mouse Neopallium -- Cultures of Oligodendroblasts from Primary Cultures of Rat Neopallium -- Olfactory Ensheathing Cell Cultures -- Cultures of Stem Cells of the Central Nervous System -- Aggregating Neural Cell Cultures -- Neural Cell Lines -- Virus Vectors for Gene Therapy of the Nervous System -- Preparation of Substrata for In Vitro Culture of Neurons -- Serum-Free Media for Neural Cell Cultures -- Development of Multiple-Well Plate Biological Assays -- Neural Cell Counting -- Assay for Neuronal Cell Migration -- Colony Cultures -- Hybridoma Cultures for Production of Antibodies -- Elimination of Cell Types from Mixed Neural Cell Cultures -- Quantification of Cells in Culture -- Tips for Tissue Culture.
520			_aIn Protocols for Neural Cell Culture, Third Ed., Sergey Fedoroff and Arleen Richardson extensively revise, update, and expand their best-selling and highly praised collection of readily reproducible neural tissue culture protocols. This 3rd edition adds 11 chapters describing important new procedures for the isolation, growth, and characterization of neural stem cells and for the manipulation of glial progenitor cells, as well as essential procedures for hippocampal and microglial slice cultures and transfection of neurons in culture with adenovirus. It includes key techniques for the preparation of substrata, the use of serum-free media, maintaining hybridomas, and the production and purification of monoclonal antibodies. For scientists not trained in neuroanatomy, but faced with dissecting the brain and spinal cord, most chapters in the 3rd edition provide fully detailed dissection procedures. Protocols for Neural Cell Culture, Third Ed. is a richly augmented updating of the tried and tested laboratory procedures that have made earlier editions an indispensable reference and guide to neural cell culture. Its unique wealth of practical detail on a wide range of tissue culture systems having many applications ensure that this new edition will remain an essential resource for all investigators using cell culture methodology in studying the brain and its disorders.
988			_aSpringer_Protocols_2001
650		7	_2embne _9160713 _aDesarrollo neurológico
650		7	_2embne _9672141 _aTejido nervioso
776	0	8	_iPrinted edition: _z9780896039025
776	0	8	_iPrinted edition: _z9781489941992
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1385/1592592074 _zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
942			_2lcc _cLE
998			_b06/2023 _dz _eb _zSI