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008			210730s2021 xxua o |||| 0|eng d
020			_a9781071616574
024	7		_a10.1007/978-1-0716-1657-4 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC _dES-MaUEC
050		4	_aQH440 _b2021 EB
245	0	0	_aCRISPR-Cas Methods _nVolume 2 _cedited by M. Tofazzal Islam, Kutubuddin Ali Molla
250			_a1st edition 2021
264		1	_aNew York, NY _bSpringer International Publising _c2021
300			_a1 recurso en línea (XVII, 397 páginas) _b118 ilustraciones, 106 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aSpringer Protocols Handbooks _x1949-2456
505	0		_aA Complete Methodology for the Instruction of CRISPR-based Gene Editing Using a Simplified Cell-free Extract System with Genetic Readout in Bacteria -- In silico Analysis of gRNA Secondary Structure to Predict Its Efficacy for Plant Genome Editing -- Efficient Multiplexed CRISPR-Cas12a Genome Editing in Plants -- CRISPR-dCas9 Based Targeted Manipulation of DNA Methylation in Plants -- Rapid Assembly of Multiplex Natural CRISPR Arrays -- Assembly and Assessment of Prime Editing Systems for Precise Genome Editing in Plants -- Designing, Performing and Analysing CRISPR-Cas9-mediated Genome Editing Experiments in Leguminous Plant -- Generation of Knockout and Fragment Deletion Mutants in Soybean by CRISPR-Cas9 -- Targeted Base Editing in Soybean using a CRISPR-Cas9 Cytidine Deaminase Fusion -- Genome Editing in Potato Using a Hairy Root Transformation System -- Efficient CRISPR-Cas9-Mediated Genome Editing in Tomato -- CRISPR-Cas9-Mediated Genome Editing in Rice: A Systematic Protocol for Single and Multi-Target Vector Construction -- Generating Clonal Seeds from Hybrid Rice with CRISPR-Cas9 -- CRISPR-Cas12a-based DNA Detection for Fast Pathogen Diagnosis and GMO Test in Plants -- CRISPR-Cas9-mediated Gene Editing in Human Induced Pluripotent Stem Cells -- Cell-Type-Specific CRISPR-Cas9 System with miRNAs -- CRISPR-Cas9 gene Editing in Mammalian Cells Using LentiCRISPRv2/LentiGuide-puro Vectors -- An Approach to Proximity Ligation by T4 RNA Ligase to Screen sRNA that Regulate CRISPR-Cas systems -- Genetic Engineering of a Phage-based Delivery System for Endogenous III-A CRISPR-Cas System against Mycobacterium tuberculosis -- CRISPR-Cas9-Mediated Genome Editing in Escherichia coli Bacteriophages -- CRISPR-Cas Genome Editing in the Cellulolytic Bacterium Clostridium thermocellum -- Gene Targeting in Caenorhabditis elegans Using a Combination of Multiple sgRNAs and a Homologous Recombination-mediated Repair -- Design of Repair Templates for CRISPR-Cas9-triggered Homologous Recombination in Caenorhabditis elegans.
520			_aThis second volume provides new and updated methods detailing advancements in CRISPR-Cas technical protocols. Chapters guide readers through protocols on prime editing, base editing, multiplex editing, editing in cell-free extract, in silico analysis of gRNA secondary structure and CRISPR-diagnosis. Authoritative and cutting-edge, CRISPR-Cas Methods, Volume 2 aims to serves as a laboratory manual providing scientists with a holistic view of CRISPR-Cas methodologies and its practical application for the editing of crop plants, cell lines, nematode and microorganism. The chapter "CRISPR/Cas9-mediated gene editing in human induced pluripotent stem cells" is available open access under a Creative Commons Attribution 4.0 International License via link.springer.com.".
988			_aSpringer_Protocols_2021
650		7	_2embne _9138044 _aGenética _vManuales de laboratorio
776	0	8	_iPrinted edition: _z9781071616567
776	0	8	_iPrinted edition: _z9781071616581
776	0	8	_iPrinted edition: _z9781071616598
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-0716-1657-4 _zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
942			_2lcc _cLE
998			_b08/2023 _dz _eb _zSI