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008			210525s2021 xxua o |||| 0|eng d
020			_a9781071614372
024	7		_a10.1007/978-1-0716-1437-2 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC _dES-MaUEC
050		4	_aQH585.2 _b2021 EB
245	0	0	_aNeuronal Cell Culture : _bMethods and Protocols _cedited by Shohreh Amini, Martyn K. White
250			_a2nd edition 2021
264		1	_aNew York, NY _bSpringer International Publising _c2021
300			_a1 recurso en línea (XII, 205 páginas) _b50 ilustraciones, 42 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v2311
505	0		_aGeneral Overview of Neuronal Cell Culture -- Considerations for the use of SH-SY5Y Neuroblastoma Cells in Neurobiology -- Cultured cell line models of neuronal differentiation: NT2, PC12, SK-N-MC -- Murine teratocarcinoma-derived neuronal cultures -- Isolation and propagation of primary human and rodent embryonic neural progenitor cells and cortical neurons -- Mouse Enteric Neuronal Cell Culture -- Preparation of Neural Stem Cells and Progenitors: Neuronal Production and Grafting Applications -- Slice culture modeling of CNS viral infection -- Neurospheres and Glial cell cultures: immunocytochemistry for cell phenotyping -- Transfection of Neuronal Cultures -- Lentiviral Transduction of Neuronal Cells -- Compartmentalized Neuronal Cultures -- Quantitative assessment of neurite outgrowth over growth promoting or inhibitory substrats -- Isolation and Culture of Dorsal Root Ganglia (DRG) from Rodents -- Isolation of Primary Human and Rodent Brain Microvascular Endothelial Cells: Culturing, Characterization, and High-Efficiency Transfection -- Fetal Brain Injury Models of Fetal Alcohol Syndrome: Examination of Neuronal Morphologic Condition Using Sholl Assay. .
520			_aThis second edition volume details the latest aspects of neural cells covering the practical and theoretical considerations of each techniques involved. Chapters guide readers through a general overview of the neuronal culturing principles, cell line models for neural cells, the isolation and propagation of primary cultures, stem cells, transfection and transduction of neural cultures, and other more advanced techniques. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Practical and easy to use, Neuronal Cell Culture: Methods and Protocols, Second Edition aims to be of interest to scientists at all levels studying cell culture models for neuroscientific studies. .
988			_aSpringer_Protocols_2021
650		7	_2embne _9139475 _aCélulas _vManuales de laboratorio
650		7	_2embne _9144348 _aNeuronas _vManuales de laboratorio
776	0	8	_iPrinted edition: _z9781071614365
776	0	8	_iPrinted edition: _z9781071614389
776	0	8	_iPrinted edition: _z9781071614396
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-0716-1437-2 _zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
942			_2lcc _cLE
998			_b08/2023 _dz _eb _zSI