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008			210524s2021 xxua o |||| 0|eng d
020			_a9781071614723
024	7		_a10.1007/978-1-0716-1472-3 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC _dES-MaUEC
050		4	_aQK725 _b2021 EB
245	0	0	_aChloroplast Biotechnology : _bMethods and Protocols _cedited by Pal Maliga
250			_a2nd edition 2021
264		1	_aNew York, NY _bSpringer International Publising _c2021
300			_a1 recurso en línea (XII, 369 páginas) _b70 ilustraciones, 41 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v2317
505	0		_aThe Plastid Genomes of Flowering Plants: Essential Principles -- Plastid Gene Transcription: An Update on Promoters and RNA Polymerases -- Engineering Chloroplasts for High-Level Constitutive or Inducible Transgene Expression -- Marker-Free Transplastomic Plants by Excision of Plastid Marker Genes Using Directly Repeated DNA Sequences -- Fluorescent Labeling and Confocal Microcopy of Plastids and Stromules -- Transformation of the Plastid Genome in Tobacco: The Model System for Chloroplast Genome Engineering -- Nicotiana tabacum: An Update on PEG-Mediated Plastid Transformation -- Transformation of the Plastid Genome in Tobacco Suspension Cell Cultures -- Plastid Marker Gene Excision in the Tobacco Shoot Apex by Agrobacterium-Delivered Cre Recombinases -- Rubisco Engineering by Plastid Transformation and Protocols for Assessing Expression -- Plastid Transformation in Tomato: A Vegetable Crop and Model Species -- Stable Plastid Transformation of Petunia for Studies in Basic Science -- Plastid Transformation in Potato: An Important Source of Nutrition and Industrial Materials -- Plastid Transformation of Poplar: A Model for Perennial Trees -- Plastid Transformation in Lettuce (Lactuca sativa) by Biolistic DNA Delivery -- Plastid Transformation in Sugar Beet: An Important Industrial Crop -- A Simple Technology for Generating Marker-Free Chloroplast Transformants of the Green Alga Chlamydomonas reinhardtii -- Regulated Chloroplast Gene Expression in Chlamydomonas -- Plastid Transformation in Physcomitrella patens: An Update -- Plastid Transformation of Sporelings from the Liverwort Marchantia polymorpha L..-Rapid and Modular DNA Assembly for Transformation of Marchantia Chloroplasts.
520			_aThis second edition volume expands on the previous edition with updated chapters in model systems and new crops. The book contains protocols for plastid engineering in leaves, tissue culture cells, and the shoot apex of plants, as well as for marker excision from the plastid genome and engineering Rubisco, the key enzyme of photosynthesis. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and practical, Chloroplast Biotechnology: Methods and Protocols, Second Edition is a valuable resource for researchers who wish to enter the field, and for practitioners looking for insights of applications in agriculture, industrial biotechnology and healthcare. .
988			_aSpringer_Protocols_2021
650		7	_2embne _9146302 _aCélulas vegetales
650		7	_2embne _9140931 _aBiotecnología
776	0	8	_iPrinted edition: _z9781071614716
776	0	8	_iPrinted edition: _z9781071614730
776	0	8	_iPrinted edition: _z9781071614747
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-0716-1472-3 _zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
942			_2lcc _cLE
998			_b08/2023 _dz _eb _zSI