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020 _a9781071614297
024 7 _a10.1007/978-1-0716-1429-7
_2doi
040 _aES-MaUEC
_bspa
_cES-MaUEC
_dES-MaUEC
050 4 _aQK731
_b2021 EB
245 0 0 _aStrigolactones :
_bMethods and Protocols
_cedited by Cristina Prandi, Francesca Cardinale
250 _a1st edition 2021
264 1 _aNew York, NY
_bSpringer International Publising
_c2021
300 _a1 recurso en línea (XI, 262 páginas)
_b86 ilustraciones, 48 ilustraciones a color
336 _atexto
_btxt
_2rdacontent
337 _aelectrónico
_bc
_2rdamedia
338 _arecurso electrónico
_bcr
_2rdacarrier
347 _aarchivo de texto
_bPDF
490 0 _aMethods in Molecular Biology
_x1940-6029
_v2309
505 0 _aEvaluation and Quantification of Natural Strigolactones from Root Exudates -- Isolation and Identification of Naturally Occurring Strigolactones -- Chemical Synthesis of Triazole-Derived Suppressors of Strigolactone Functions -- Synthesis of Simple Strigolactone Mimics -- Synthesis of Strigolctones Analogues and Evaluation of their Stability in Solution -- Strigolactone-like Bioactivity Via Parasitic Plant Germination Bioassay -- Evaluation of the Effect of Strigolactones and Synthetic Analogues On Fungi -- Analysing the Effect of Strigolactones on the Motility Behaviour of Rhizobia -- Chemotropic Assay for Testing Fungal Response to Strigolactones and Strigolactone-Like Compounds -- Methods for Phenotyping Shoot Branching and Testing Strigolactone Bioactivity for Shoot Branching in Arabidopsis and Pea -- Bioassays for the Effects of Strigolactones and Other Small Molecules on Root and Root Hair Development -- Methods for Medium-Scale Study of the Biological Effects of Strigolactone-Like Molecules on the Moss Physcomitrella patens -- Controlled Assays for Phenotyping the Effects of Strigolactone-like Molecules on Arbuscular Mycorrhiza Development -- Application of Strigolactones to Plant Roots to Influence the Formation of Symbioses -- Evaluation of the Bioactivity Oof Strigolactone-Related Molecules by a Quantitative Luminometer Bioassay -- A Protoplast-based Bioassay to Quantify Strigolactone Activity in Arabidopsis using StrigoQuant -- Synthesis of Profluorescent Strigolactone Probes for Biochemical Studies -- The use of Differential Scanning Fluorimetry to Assess Strigolactone Receptor Function -- Structural Analysis of Strigolactone-Related Gene Products.
520 _aThis volume presents the most useful laboratory protocols in strigolactones(SL) research. Chapters guide readers through wet-lab paths, issues around stability, protocols to evaluate SL activity, effects towards soil inhabitants such as parasitic plants, mycorrhizal and non-mycorrhizal fungi, nodulating bacteria, and protocols to assess effects on plant development are discussed. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Strigolactones: Methods and Protocols aims to deliver a clear-cut and standardized set of experimental protocols to a broad scientific community.
988 _aSpringer_Protocols_2021
650 7 _2embne
_9668331
_aHormonas vegetales
_vManuales de laboratorio
776 0 8 _iPrinted edition:
_z9781071614280
776 0 8 _iPrinted edition:
_z9781071614303
776 0 8 _iPrinted edition:
_z9781071614310
856 4 0 _uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-0716-1429-7
_zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
942 _2lcc
_cLE
998 _b09/2023
_dz
_eb
_zSI