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008			210505s2021 xxua o |||| 0|eng d
020			_a9781071614068
024	7		_a10.1007/978-1-0716-1406-8 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC _dES-MaUEC
050		4	_aQP551 _b2021 EB
245	0	0	_aStructural Proteomics : _bHigh-Throughput Methods _cedited by Raymond J. Owens
250			_a3rd edition 2021
264		1	_aNew York, NY _bSpringer International Publising _c2021
300			_a1 recurso en línea (XII, 344 páginas) _b88 ilustraciones, 67 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v2305
505	0		_aThe Protein Data Bank Archive -- Computational Methods for the Elucidation of Protein Structure and Interactions -- Methods for Molecular Modeling of Protein Complexes -- High-Level Production of Recombinant Eukaryotic Proteins from Mammalian Cells Using Lentivirus -- Transient Transfection and Expression of Eukaryotic Membrane Proteins in Expi293F Cells and Their Screening on a Small-Scale: Application for Structural Studies -- Reproducible and Easy Production of Mammalian Proteins by Transient Gene Expression in High Five Insect Cells -- SynBac: Enhanced Baculovirus Genomes by Iterative Recombineering -- Gene Tagging with the CRISPR-Cas9 System to Facilitate Macromolecular Complex Purification -- Synthesis of Fluorescently Labeled Antibodies Using Non-Canonical Amino Acids in Eukaryotic Cell-Free Systems -- Solid-State NMR Spectroscopy for Studying Microtubules and Microtubule-Associated Proteins -- Dynamic Structural Biology Experiments at XFEL or Synchrotron Sources -- From Tube to Structure: SPA Cryo-EM Workflow Using Apoferritin as an Example -- Image Processing in Cryo-Electron Microscopy of Single Particles: The Power of Combining Methods -- Setup and Troubleshooting of Volta Phase Plate Cryo-EM Data Collection -- Cryo-Focused Ion Beam Lamella Preparation Protocol for In Situ Structural Biology -- Protein and Small Molecule Structure Determination by the Cryo-EM Method MicroED.
520			_aThis updated and expanded volume reflects the current state of the structural protein field with improved and refined protocols that have been applied to particularly challenging proteins. Beginning with a section on structural bioinformatics, the book continues with sections covering the challenge of producing high quality samples for structural studies, particularly mammalian membrane proteins and protein complexes, as well as protocols for structure determination, including the use of electrons in structural biology and more. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and up-to-date, Structural Proteomics: High-Throughput Methods, Third Edition will aid researchers in expanding our knowledge of this vital and expansive area of protein science. Chapter 9 is available open access under a CC BY 4.0 license.
988			_aSpringer_Protocols_2021
650		7	_2embne _9430647 _aProteómica
776	0	8	_iPrinted edition: _z9781071614051
776	0	8	_iPrinted edition: _z9781071614075
776	0	8	_iPrinted edition: _z9781071614082
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-0716-1406-8 _zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
942			_2lcc _cLE
998			_b08/2023 _dz _eb _zSI