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008			210429s2021 xxua o |||| 0|eng d
020			_a9781071613115
024	7		_a10.1007/978-1-0716-1311-5 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC _dES-MaUEC
050		4	_aQR185.8 .T2 _b2021 EB
245	0	0	_aT-Helper Cells : _bMethods and Protocols _cedited by Francesco Annunziato, Laura Maggi, Alessio Mazzoni
250			_a1st edition 2021
264		1	_aNew York, NY _bSpringer International Publising _c2021
300			_a1 recurso en línea (XII, 388 páginas) _b103 ilustraciones, 51 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v2285
505	0		_aHuman and Murine T helper Cell Recovery from Organs and Tissues -- Identification and Purification of Human Memory T Helper Cells from Peripheral Blood -- Transcription Factor Evaluation by Flow Cytometry -- Mass Cytometry Analysis of T Helper Cells -- Murine T Helper Cell Differentiation and Plasticity -- Identification of Follicular T Cell Subsets in Murine and Human Tissues -- Analysis of T Cells In Inflamed Non-Lymphoid Tissues -- TCR Vβ Evaluation by Flow Cytometry -- Evaluation of Cytokine Production at the Single Cell Level by Flow Cytometry Upon Polyclonal Stimulation -- Evaluation of Secreted Cytokines by Multiplex Bead Based Assay (X MAP Technology, Luminex) -- Quantifying Antigen-specific T Cells by Assessing their Antigen-Induced Proliferation -- Flow Cytometric Characterization of Human Antigen-Reactive T helper Cells -- Identification of Human Antigen-Specific CD4+ cells with Peptide-MHC Multimer Technologies -- Human T cell Cloning by Limiting Dilution -- Human Germinal Center Reaction: Assessing T Follicular Helper-B cell Interactions In Vitro -- Study of Signal Transduction Pathways by Phospho-Protein Evaluation -- The Study of Protein-DNA Interactions in CD4+ T Cells using ChIPmentation -- mRNA Transfection of T lymphocytes by Electroporation -- Transduction of Human T Cell Subsets with Lentivirus -- Plasmid- or Ribonucleoprotein-mediated CRISPR/Cas Gene Editing in Primary Murine T Cells -- Generation of Sequencing Libraries for Building Immune Cell Methylomes -- Gene Expression Analysis of T Cells by Single Cell RNA-seq -- Quantifying Proteome and Protein Modifications in Activated T Cells by Multiplexed Isobaric Labeling Mass Spectrometry -- Metabolomics, Lipidomics and Immunometabolism -- Experimental Mouse Models of Asthma and Analysis of CD4+ T Cells -- Murine T Cell Transfer Colitis as a Model for Inflammatory Bowel Disease -- Experimental Autoimmune Encephalomyelitis.
520			_aThe aim of this volume is to provide a comprehensive description of methods and protocols useful for the further study of T-helper cells. Chapters guide readers through T-helper cell recovery, molecular study, signal transduction pathways, T-cell manipulation and, last but not least, "omic" approaches. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, T- Helper Cells: Methods and Protocols aims to be a useful practical guide to researches to help further their study in this field.
988			_aSpringer_Protocols_2021
650		7	_2embne _9144344 _aLinfocitos T _vManuales de laboratorio
650		7	_2embne _9138330 _aInmunología _vManuales de laboratorio
776	0	8	_iPrinted edition: _z9781071613108
776	0	8	_iPrinted edition: _z9781071613122
776	0	8	_iPrinted edition: _z9781071613139
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-0716-1311-5 _zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
942			_2lcc _cLE
998			_b08/2023 _dz _eb _zSI