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008			210420s2021 xxu| o |||| 0|eng d
020			_a9781071613948
024	7		_a10.1007/978-1-0716-1394-8 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC _dES-MaUEC
050		4	_aQP552 .M44 _b2021 EB
245	0	0	_aStructure and Function of Membrane Proteins _cedited by Ingeborg Schmidt-Krey, James C. Gumbart
250			_a1st edition 2021
264		1	_aNew York, NY _bSpringer International Publising _c2021
300			_a1 recurso en línea (XII, 358 páginas) _b97 ilustraciones, 70 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v2302
505	0		_aExpression and Purification of Human Mitochondrial Intramembrane Protease PARL -- Reconstitution of Detergent-Solubilized Membrane Proteins into Proteoliposomes and Nanodiscs for Functional and Structural Studies -- Biochemical Characterization of GPCR-G Protein Complex Formation -- Electrophysiological Approaches for the Study of Ion Channel Function -- Isothermal Titration Calorimetry of Membrane Proteins -- Atomic Force Microscopy Reveals Membrane Protein Activity at the Single Molecule Level -- Structure Determination of Membrane Proteins Using X-Ray Crystallography -- Studying Membrane Protein Structures by MicroED -- Single-Particle Cryo-EM of Membrane Proteins -- Helical Membrane Protein Crystallization in the New Era of Electron Cryo-Microscopy -- NMR Spectroscopic Studies of Ion Channels in Lipid Bilayers: Sample Preparation Strategies Exemplified by the Voltage Dependent Anion Channel -- Preparation of a Deuterated Membrane Protein for Small-Angle Neutron Scattering -- Preparing Membrane Proteins for Simulation Using CHARMM-GUI -- Coarse-Grained Molecular Dynamics Simulations of Membrane Proteins: A Practical Guide -- Continuous Constant pH Molecular Dynamics Simulations of Transmembrane Proteins -- Molecular Dynamics-Based Thermodynamic and Kinetic Characterization of Membrane Protein Conformational Transitions -- Concepts, Practices, and Interactive Tutorial for Allosteric Network Analysis of Molecular Dynamics Simulations -- Large Scale Molecular Dynamics Simulations of Cellular Compartments.
520			_aThis book examines detailed experimental and computational approaches for the analysis of many aspects vital to the understanding of membrane protein structure and function. Readers will receive guidance on the selection and use of methods for over-expression and purification, tools to characterize membrane proteins within different phospholipid bilayers, direction on functional studies, and approaches to determine the structures of membrane proteins. Detailed experimental steps for specific membrane proteins with critical notes allow the protocols to be modified to different systems. Written for the highly successful Methods in Molecular Biology series, chapters include the kind of practical information and implementation advice that leads to excellent, reproducible results. Authoritative and up-to-date, Structure and Function Studies of Membrane Proteins serves as an ideal guide for biologists, biochemists, and biophysicists striving to further understand these essential proteins and their many biological roles.
988			_aSpringer_Protocols_2021
650		7	_2embne _9671893 _aProteínas de membranas _vManuales de laboratorio
776	0	8	_iPrinted edition: _z9781071613931
776	0	8	_iPrinted edition: _z9781071613955
776	0	8	_iPrinted edition: _z9781071613962
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-0716-1394-8 _zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
942			_2lcc _cLE
998			_b08/2023 _dz _eu _zSI