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008			210219s2021 xxu| o |||| 0|eng d
020			_a9781071610084
024	7		_a10.1007/978-1-0716-1008-4 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC _dES-MaUEC
050		4	_aQH442.6 _b2021 EB
245	0	0	_aMouse Genetics : _bMethods and Protocols _cedited by Shree Ram Singh, Robert M. Hoffman, Amit Singh
250			_a2nd edition 2021
264		1	_aNew York, NY _bSpringer International Publising _c2021
300			_a1 recurso en línea (XI, 217 páginas) _b47 ilustraciones, 41 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v2224
505	0		_aGeneration of Mouse Model (KI & CKO) via Easi-CRISPR -- Analysis of Gene Expression Using lacZ Reporter Mouse Lines -- Linear Density Sucrose Gradients to Study Mitoribosomal Biogenesis in Tissue-Specific Knockout Mice -- Mouse Models for Studying Hippocampal Adult Neural Stem Cell Biology -- Retina as a Model to Study In Vivo Transmission of α-Synuclein in the A53T Mouse Model of Parkinson's Disease -- Promoting Pro-Endocrine Differentiation and Graft Maturation Following Surgical Resection of the Mouse Pancreas -- Color-Coded Imaging of Cancer and Stromal Cells Interaction in the Pancreatic Cancer Tumor Microenvironment (TME) -- Generating Ins2+/-/miR-133aTg Mice to Model miRNA-Driven Cardioprotection of Diabetic Hearts in Humans -- Generating a Podocyte-Specific FcRn Knockout Mouse -- Mouse Models of Colitis-Associated Colon Cancer -- Colon Cancer Tumor Model Base on Colonoscopy Injection -- Generation of Transgenic Fluorescent Reporter Lines for Studying Hematopoietic Development in the Mouse -- Gene Inactivation in Adult Long Term-Hematopoietic Stem Cells Using Inducible Mouse Models -- Hematological Humanization of Immune Deficient Mice -- Antisense Oligonucleotide Treatment in a Humanized Mouse Model of Duchenne Muscular Dystrophy and Highly Sensitive Detection of Dystrophin Using Western Blotting.
520			_aThis fully updated edition provides selected mouse genetic techniques and their application in modeling varieties of human diseases. The chapters are mainly focused on the generation of different transgenic mice to accomplish the manipulation of genes of interest, tracing cell lineages, and modeling human diseases. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and up-to-date, Mouse Genetics: Methods and Protocols, Second Edition delivers fundamental techniques and protocols to geneticists, molecular biologists, cell and developmental biologists, students, and postdoctoral fellows working in the various disciplines of genetics, developmental biology, mouse genetics, and modeling human diseases.
988			_aSpringer_Protocols_2021
650		7	_2embne _9155966 _aOrganismos transgénicos _vManuales de laboratorio
776	0	8	_iPrinted edition: _z9781071610077
776	0	8	_iPrinted edition: _z9781071610091
776	0	8	_iPrinted edition: _z9781071610107
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-0716-1008-4 _zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
942			_2lcc _cLE
998			_b08/2023 _dz _eu _zSI