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008			210213s2020 xxu| o |||| 0|eng d
020			_a9781071607244
024	7		_a10.1007/978-1-0716-0724-4 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC _dES-MaUEC
050		4	_aQP552 .M44 _b2021 EB
245	0	0	_aBiophysics of Membrane Proteins : _bMethods and Protocols _cedited by Vincent L. G. Postis, Adrian Goldman
250			_a1st edition 2020
264		1	_aNew York, NY _bSpringer International Publising _c2020
300			_a1 recurso en línea (XI, 336 páginas) _b73 ilustraciones, 61 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v2168
505	0		_aMembrane Protein Production and Purification from Escherichia coli and Sf9 Insect Cells -- Quantifying the Interaction of Phosphite with ABC Transporters: MicroScale Thermophoresis and a Novel His-tag Labeling Approach -- Rationale for the Quantitative Reconstitution of Membrane Proteins into Proteoliposomes -- Functional Characterization of SLC transporters using Solid-Supported-Membranes -- Thermostability Assays Provide a Generic and Versatile Tool for Studying the Functional and Structural Properties of Membrane Proteins in Detergents -- Direct monitoring of GPCR reconstitution and ligand binding activity by Plasmon Waveguide Resonance -- Examining Membrane Proteins by Neutron Scattering -- Solution X-ray Scattering for Membrane Protein -- Interpreting SAXS/WAXS Data with Explicit-Solvent Simulations: A Practical Guide -- Determining the Free Energies of Outer Membrane Proteins in Lipid Bilayers -- Interrogating Membrane Protein Structure and Lipid Interactions by Native Mass Spectrometry -- Determination of the Molecular Mass of Membrane Proteins Using Size Exclusion Chromatography with Multi-angle Laser Light Scattering (SEC-MALLS) -- Dynamics of Membrane Proteins Monitored by Single-molecule Fluorescence across Multiple Time Scale -- Short-Range Distances Measurement by Transition Metal Ion FRET -- PELDOR/DEER - An Electron Paramagnetic Resonance Method to Study Membrane Proteins in Lipid Bilayers. .
520			_aThis volume provides recent advances in the field of biophysics of membrane proteins. Chapters are divided into several parts: detailing biochemistry and functional analysis, experimental and theoretical structural determinations, membrane protein dynamics, and conformation studies. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Biophysics of Membrane Proteins: Methods and Protocols aims to provide comprehensive protocols with notes to help further the understanding of key membrane protein structure and function for students, academics, and industrial researchers.
988			_aSpringer_Protocols_2020
650		7	_2embne _9671893 _aProteínas de membranas _vManuales de laboratorio
776	0	8	_iPrinted edition: _z9781071607237
776	0	8	_iPrinted edition: _z9781071607251
776	0	8	_iPrinted edition: _z9781071607268
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-0716-0724-4 _zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
942			_2lcc _cLE
998			_b08/2023 _dz _eu _zSI