000			04123nam a22003975i 4500
999			_c391435 _d391435
001			391435
003			ES-MaUEC
005			20230804142125.0
006			a||||fo|||| 00| 0
007			cr nn 008mamaa
008			210123s2021 xxu| o |||| 0|eng d
020			_a9781071610954
024	7		_a10.1007/978-1-0716-1095-4 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC _dES-MaUEC
050		4	_aQP95 _b2021 EB
245	0	0	_aEosinophils : _bMethods and Protocols _cedited by Garry M. Walsh
250			_a2nd edition 2021
264		1	_aNew York, NY _bSpringer International Publising _c2021
300			_a1 recurso en línea (XII, 297 páginas) _b65 ilustraciones, 51 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v2241
505	0		_aMolecular Biology of Eosinophils: Introduction -- Functionally Active Eosinophil Purification from Peripheral Blood -- Eosinophil Purification from Peripheral Blood of Rhesus Monkeys -- Generation of Mouse Eosinophils in Tissue Culture from Unselected Bone Marrow Progenitors -- Detection of Mouse Eosinophils in Tissue by Flow Cytometry and Isolation by Fluorescence-Activated Cell Sorting (FACS) -- Mutant Mice and Animal Models of Airway Allergic Disease -- Eosinophils in Asthma Models to House Dust Mite for Drug Development -- Analysis of Mouse Eosinophil Migration and Killing of Tumor Cells -- Murine Models of Eosinophil Function in Fungal and Viral Infections -- Assays of Eosinophil Apoptosis and Phagocytic Uptake -- In Vivo Eosinophil Expansion Using Gene Transfer by Electroporation -- Observation and Quantification of Eosinophil Motility -- Isolation and Functional Aspects of Eosinophil-Derived Exosomes -- Interactions of Eosinophils with Nerves -- Measurement of Eosinophil Kinetics In Vivo -- Detection of Eosinophil Extracellular DNA Traps -- Eosinophil Shape Change and Secretion -- Update on Eosinophils Interaction with Mast Cells: The Allergic Effector Unit -- Assessing Phenotypic Heterogeneity in Intestinal Tissue Eosinophils -- Antimicrobial Activity of Human Eosinophil Granule Proteins -- Experimental Modeling of Eosinophil-Associated Diseases.
520			_aThis second edition of Eosinophils: Methods and Protocols updates several techniques from the first edition together with novel in vitro and in vivo-based methodologies. Written by internationally recognized expert authorities, this volume provides vital techniques from eosinophil purification to experimental modelling, with each technique spelled out in clear and straightforward terms, assuming no previous knowledge of the method and without necessitating sourcing additional information elsewhere. Written in the highly successful Methods in Molecular Biology format, chapters include introductions to their respective topics, lists of the essential materials and reagents, step-by-step, readily reproducible laboratory protocols, with tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Eosinophils: Methods and Protocols, Second Edition provides the practical means to extend our knowledge of eosinophil function in health and disease, underpinning research that may in turn lead to new hypotheses for future examinations into the role of this intriguing and enigmatic leukocyte. Chapters 10 is available open access under a Creative Commons Attribution 4.0 International License via link.springer.com.
988			_aSpringer_Protocols_2021
650		7	_2embne _9160868 _aCélulas sanguíneas _vManuales de laboratorio
776	0	8	_iPrinted edition: _z9781071610947
776	0	8	_iPrinted edition: _z9781071610961
776	0	8	_iPrinted edition: _z9781071610978
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-0716-1095-4 _zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
942			_2lcc _cLE
998			_b08/2023 _dz _eu _zSI