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020			_a9781071611425
024	7		_a10.1007/978-1-0716-1142-5 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC _dES-MaUEC
050		4	_aQP752 .P52 _b2021 EB
245	0	0	_aPhosphoinositides : _bMethods and Protocols _cedited by Roberto J. Botelho
250			_a1st edition 2021
264		1	_aNew York, NY _bSpringer International Publising _c2021
300			_a1 recurso en línea (XI, 239 páginas) _b50 ilustraciones, 43 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v2251
505	0		_aSimultaneous Detection of Phosphoinositide Lipids by Radioactive Metabolic Labeling -- Label-Free Quantification of Phosphoinositides in Drosophila by Mass Spectrometry -- Profiling of Phosphoinositide Molecular Species in Resting or Activated Human or Mouse Platelets by a LC-MS Method -- Quantification of Genetically-Encoded Lipid Biosensors -- Detection of Plasma Membrane Phosphoinositide Dynamics Using Genetically-Encoded Fluorescent Protein Probes -- Imaging the Nanoscale Distribution of Phosphoinositides in the Cell Plasma Membrane with Single-Molecule Localization Super-Resolution Microscopy -- Induced Dimerization Tools to Deplete Specific Phosphatidylinositol Phosphates -- A Real-Time Phosphatidylinositol 4-Phosphate 5-Kinase Assay Using Fluorescence Spectroscopy -- Assessing In Situ Phosphoinositide-Protein Interactions through Fluorescence Proximity Ligation Assay in Cultured Cells -- Characterization of Protein-Phospholipid/Membrane Interactions Using a "Membrane-on-a-Chip" Microfluidic System -- Exploring Phosphoinositide Binding Using Native Mass Spectrometry -- Liposome-Based Methods to Study Protein-Phosphoinositide Interaction -- Liposome-Based Methods to Study GTPases Activation by Phosphoinositides -- Liposome Co-Sedimentation and Co-Flotation Assays to Study Lipid-Protein Interactions -- Characterization of PROPPIN-Phosphoinositide Binding by Stopped-Flow Fluorescence Spectroscopy -- Fluorescence Assays to Study Membrane Penetration of Proteins -- Fluorogenic XY-69 in Lipid Vesicles for Measuring Activity of Phospholipase C Isozymes.
520			_aThis detailed book examines experimental approaches used to investigate the regulation and function of phosphoinositides (PtdInsP), rare eukaryotic phospholipids with a broad role in biological processes such as signal transduction, cell migration and adhesion, cell growth, subcellular organization, and membrane trafficking. The combination of complementary biochemical, mass spectrometry, and imaging methods are instrumental for the detection and quantification of PtdInsP species, as well as induced dimerization methods, affinity precipitation or co-sedimentation with liposomes, protein insertion within lipid bilayers, and enzymatic assays, or through emerging methods like native mass spectrometry and microfluidics, all of which are touched upon in this volume. Written in the highly successful Methods in Molecular Biology format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Phosphoinositides: Methods and Protocols provides detailed methodology for both specialist and novice researchers on a variety of complementary methods that have been instrumental in dissecting the regulation, dynamics, and function of PtdInsPs.
988			_aSpringer_Protocols_2021
650		7	_2embne _9143582 _aFosfolípidos _vManuales de laboratorio
776	0	8	_iPrinted edition: _z9781071611418
776	0	8	_iPrinted edition: _z9781071611432
776	0	8	_iPrinted edition: _z9781071611449
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-0716-1142-5 _zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
942			_2lcc _cLE
998			_b08/2023 _dz _eu _zSI