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008			210120s2021 xxu| o |||| 0|eng d
020			_a9781071610688
024	7		_a10.1007/978-1-0716-1068-8 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC _dES-MaUEC
050		4	_aSB191 .R5 _b2021 EB
245	0	0	_aRice Genome Engineering and Gene Editing : _bMethods and Protocols _cedited by Anindya Bandyopadhyay, Roger Thilmony
250			_a1st edition 2021
264		1	_aNew York, NY _bSpringer International Publising _c2021
300			_a1 recurso en línea (XV, 378 páginas) _b81 ilustraciones, 70 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v2238
505	0		_aGene Assembly in Agrobacterium via Nucleic Acid Transfer Using Recombinase Technology (GAANTRY) -- TransGene Stacking II Vector System for Plant Metabolic Engineering and Synthetic Biology -- Morphogenic Regulators and Their Application in Improving Plant Transformation -- A Rapid Method for Stably Transforming Rice Seeds -- Agrobacterium tumefaciens-Mediated Transformation of Rice by Hygromycin Phosphotransferase (hptII) Gene Containing CRISPR/Cas9 Vector -- Genotype Independent Regeneration and Transformation Protocol for Rice Cultivars -- Rapid Vector Construction and Assessment of BE3 and Target-AID C to T Base Editing Systems in Rice Protoplasts -- Genome Editing of Rice by CRISPR-Cas: End-to-End Pipeline for Crop Improvement -- Single Base Editing Using Cytidine Deaminase to Change Grain Size and Seed Coat Color in Rice -- Analysis of Off-Target Mutations in CRISPR-Edited Rice Plants Using Whole Genome Sequencing -- Efficient Genome Editing in Rice Protoplasts Using CRISPR/CAS9 Construct -- Single Transcript Unit CRISPR 2.0 Systems for Genome Editing in Rice -- Improving a Quantitative Trait in Rice by Multigene Editing with CRISPR-Cas9 -- Rice Haploid Inducer Development by Genome Editing -- FLP-Mediated Site-Specific Gene Integration in Rice -- Rice Gene Targeting by Homologous Recombination with Positive Negative Selection Strategy -- Prediction of Rice Transcription Start Sites Using TransPrise: A Novel Machine Learning Approach -- Single Cell Type Specific RNA Isolation and Gene Expression Analysis in Rice Using Laser Capture Microdissection (LCM)-Based Method -- Immunolocalization Analysis of C4 Proteins in the Leaf Tissue of Rice -- Selection of Suitable Reference Genes for qRT-PCR Gene Expression Studies in Rice -- Rice Protoplast Isolation and Transfection for Transient Gene Expression Analysis -- Identification and Analysis of Steroidogenic Acute Regulatory (StAR) Protein-Related Lipid Transfer (START) Domains in Rice -- Assessing Rice Salinity Tolerance: From Phenomics to Association Mapping.
520			_aThis detailed volume explores rice molecular biology, genetic engineering, and genome editing technologies. Dividing into three parts, the book covers subjects such as genetic engineering and tissue culture of rice, including efficient methods for rice transformation and regeneration, genome editing, targeted integration, and gene stacking in rice, including multiple methods utilizing CRISPR systems for targeted gene knock-out or genome modification via base editing, and diverse methods describing bioinformatic, molecular, and cellular analyses in rice. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Rice Genome Engineering and Gene Editing: Methods and Protocols serves as a valuable resource for researchers worldwide striving to further their efforts on advancing research and producing genetically improved rice varieties.
988			_aSpringer_Protocols_2021
650		7	_2embne _9138676 _aArroz _vManuales de laboratorio
650		7	_2embne _9139640 _aGenética vegetal _vManuales de laboratorio
776	0	8	_iPrinted edition: _z9781071610671
776	0	8	_iPrinted edition: _z9781071610695
776	0	8	_iPrinted edition: _z9781071610701
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-0716-1068-8 _zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
942			_2lcc _cLE
998			_b08/2023 _dz _eu _zSI