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008			210109s2021 xxu| o |||| 0|eng d
020			_a9781071610916
024	7		_a10.1007/978-1-0716-1091-6 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC _dES-MaUEC
050		4	_aRA1199 _b2021 EB
245	1	0	_aToxicity Assessment _bMethods and Protocols _cedited by Carlos Manuel Marques Palmeira, Danielle Palma de Oliveira, Daniel Junqueira Dorta.
250			_a1st edition 2021
264		1	_aNew York, NY _bSpringer International Publising _c2021
300			_a1 recurso en línea (XII, 266 páginas) _b78 ilustraciones, 68 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v2240
505	0		_aThe Single Cell Gel Electrophoresis Genotoxin Sensitivity Assay -- Skin Sensitization Tests: The LLNA and the RhE IL-18 Potency Assay -- Skin Equivalent Models: Protocols for In Vitro Reconstruction for Dermal Toxicity Evaluation -- Intravital Microscopy: A Tool to Investigate the Toxicity in the Immune System, Vessel Rheology, and Xenobiotic Distribution -- Cell-Based Biosensor to Visualize Nitric Oxide Release from Living Cells for Toxicity Assessment -- In Vitro Contraction of Isolated Cauda Epididymal Duct Smooth Muscle as a Complimentary Approach to Physiological, Pathological, Toxicological, and Pharmacological Studies on Epididymal Function -- Early Life Stage Assays in Zebrafish -- Assessment of Urothelial Cytotoxicity at Morphological and Molecular Levels: Urinary Bladder as Target Organ -- Evaluation of Development of the Rat Uterus as a Toxicity Biomarker -- Acetylcholinesterase (AChE) Activity in Embryos of Zebrafish -- Ecotoxicity Assays Using Freshwater Planarians -- Toxicogenomic Analysis -- Strategy Combining Non-Animal Methods for Ocular Toxicity Evaluation -- Immunocytochemistry Analysis of HepG2 Cell 3D Culture Encapsulated as Spheroids in Alginate Beads -- Oxygen Consumption Evaluation: An Important Indicator of Metabolic State, Cellular Function and Cell Fate Along Neural Deregulation -- Mitochondrial Bioenergetic Assays as a Standard Protocol for Toxicological and Metabolic Assessment -- In Vitro Apoptotic Cell Death Assessment.
520			_aThis book collects protocols from different areas of knowledge to assist in the identification of toxic effects exerted by different xenobiotics. At the same time as classical techniques are presented, modern techniques with alternative models to the use of animals are also presented. Given the ever-increasing exposure to different compounds and their effects on population health, the assessment of multiple endpoints is of utmost importance for better risk assessment, and this collection addresses that need. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Toxicity Assessment: Methods and Protocols aims to serve researchers in this vast field of science as they seek to better understand the mechanisms of action of different xenobiotics.
988			_aSpringer_Protocols_2021
650		7	_2embne _9140328 _aToxicología _vManuales de laboratorio
776	0	8	_iPrinted edition: _z9781071610909
776	0	8	_iPrinted edition: _z9781071610923
776	0	8	_iPrinted edition: _z9781071610930
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-0716-1091-6 _zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
942			_2lcc _cLE
998			_b08/2023 _dz _eu _zSI