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008			201226s2021 xxu| o |||| 0|eng d
020			_a9781071609668
024	7		_a10.1007/978-1-0716-0966-8 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC _dES-MaUEC
050		4	_aQH212 .E4 _b2021 EB
245	0	0	_acryoEM : _bMethods and Protocols _cedited by Tamir Gonen, Brent L. Nannenga
250			_a1st edition 2021
264		1	_aNew York, NY _bSpringer International Publising _c2021
300			_a1 recurso en línea (XI, 350 páginas) _b89 ilustraciones, 74 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v2215
505	0		_aGetting Started with in situ cryo-electron Tomography -- Cryo-Electron Tomography and Automatic Segmentation of Cultured Hippocampal Neurons -- Practical Approaches for Cryo-FIB Milling and applications for Cellular Cryo-Electron Tomography -- In situ Imaging and Structure Determination of Biomolecular Complexes using Cryo-Electron Tomography -- Progress Towards CryoEM: Negative-stain Procedures for Biological Samples -- Setting up Parallel Illumination on the Talos Arctica for High-Resolution Data Collection -- Multi-body refinement of cryo-EM images in RELION -- Evaluating Local and Directional Resolution of Cryo-EM Density Maps -- Automated modeling and Validation of Protein Complexes in cryo-EM Maps -- 2D Electron Crystallography of Membrane Protein Single-, Double- and Multi-Layered Ordered Arrays -- Sample Preparation and Data Collection for Electron Crystallographic Studies on Membrane Protein Structures and Lipid-Protein Interaction -- Single Particle Analysis for High Resolution 2D Electron Crystallography -- MicroED Sample Preparation and Data Collection for Protein Crystals.-- Detection of Microcrystals for CryoEM -- Low-dose Data Collection and Radiation Damage in MicroED -- Automation of Continuous-rotation Data Collection for MicroED -- Ab initio Determination of Peptide Structures by MicroED. .
520			_aThis volume details the most up-to-date cryo-EM techniques from leading researchers. Chapters are organized into four parts with emphasis on electron cryotomography, single particle analysis, and the crystal based cryo-EM methods of 2D electron crystallography, and MicroED for the study of 3D crystals. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, application details for both the expert and non-expert reader, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, CryoEM: Methods and Protocols aims to serve as an excellent resource on cryo-EM and can serve as the foundation for new researchers to this growing field in structural biology. .
988			_aSpringer_Protocols_2021
650		7	_2embne _9138721 _aMicroscopia electrónica _vManuales de laboratorio
776	0	8	_iPrinted edition: _z9781071609651
776	0	8	_iPrinted edition: _z9781071609675
776	0	8	_iPrinted edition: _z9781071609682
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-0716-0966-8 _zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
942			_2lcc _cLE
998			_b08/2023 _dz _eu _zSI