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008			201219s2021 xxu| o |||| 0|eng d
020			_a9781071611746
024	7		_a10.1007/978-1-0716-1174-6 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC _dES-MaUEC
050		4	_aQH491 _b2021 EB
245	0	0	_aProgrammed Morphogenesis : _bMethods and Protocols _cedited by Mo R. Ebrahimkhani, Joshua Hislop
250			_a1st edition 2021
264		1	_aNew York, NY _bSpringer International Publising _c2021
300			_a1 recurso en línea (XII, 286 páginas) _b70 ilustraciones, 69 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v2258
505	0		_aTASBE Image Analytics: A Processing Pipeline for Quantifying Cell Organization from Fluorescent Microscopy -- Neighborhood Impact Factor to Study Cell-Fate Decision-Making in Cellular Communities -- A Quantitative Lineage-Tracing Approach to Understand Morphogenesis in Gut -- Reconstitution of Morphogen Signaling Gradients in Cultured Cells -- Engineering Shape-Controlled Microtissues on Compliant Hydrogels with Tunable Rigidity and Extracellular Matrix Ligands -- Engineering Biophysical Cues for Controlled 3D Differentiation of Endoderm Derivatives -- Rewiring Endogenous Bioelectric Circuits in the Xenopus laevis Embryo Model -- Engineering the Spatiotemporal Mosaic Self-Patterning of Pluripotent Stem Cells -- Fate-Patterning of 2D Gastruloids and Ectodermal Colonies Using Micropatterned Human Pluripotent Stem Cells -- Gastruloids: Embryonic Organoids from Mouse Embryonic Stem Cells to Study Patterning and Development in Early Mammalian Embryos -- A Synergistic Engineering Approach to Build Human Brain Spheroids -- Directed Differentiation of Human Pluripotent Stem Cells for the Generation of High-Order Kidney Organoids -- Methods for Controlled Induction of Singular Rosette Cytoarchitecture within Human Pluripotent Stem Cell-Derived Neural Multicellular Assemblies -- 3D Self-Organized Human Blood-Brain Barrier in a Microfluidic Chip -- Modeling the Complexity of the Metastatic Niche Ex Vivo -- Fabrication Method of a High-Density Co-Culture Tumor-Stroma Platform to Study Cancer Progression -- A Method for Organoid Transplantation and Whole-Mount Visualization of Post-Engraftment Vascularization -- High Throughput Production of Platelet-Like Particles.
520			_aThis detailed book explores techniques for understanding and engineering programs that naturally control and drive formation of tissues and organs in order to open powerful opportunities to produce physiologically relevant tissues of interest, generate models to study human disease, and set the path for the manufacturing of advanced tissue and organs. Beginning with chapters to help understand signaling events and patterns in morphogenetic systems, the volume continues by covering programming signaling events and patterns to drive morphogenesis, techniques for engineering organoids, tissue barriers, and disease models, as well as in vivo therapeutic applications. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Programmed Morphogenesis: Methods and Protocols aims not only to communicate knowledge but also to inspire approaches to new challenges and to empower readers with the capability to approach those challenges.
988			_aSpringer_Protocols_2021
650		7	_2embne _9688497 _aMorfogénesis _vManuales de laboratorio
776	0	8	_iPrinted edition: _z9781071611739
776	0	8	_iPrinted edition: _z9781071611753
776	0	8	_iPrinted edition: _z9781071611760
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-0716-1174-6 _zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
942			_2lcc _cLE
998			_b08/2023 _dz _eu _zSI