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008			201217s2021 xxu| o |||| 0|eng d
020			_a9781071609439
024	7		_a10.1007/978-1-0716-0943-9 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC _dES-MaUEC
050		4	_aRS199.5 _b2021 EB
245	0	0	_aBio-Carrier Vectors : _bMethods and Protocols _cedited by Kumaran Narayanan
250			_a1st edition 2021
264		1	_aNew York, NY _bSpringer International Publising _c2021
300			_a1 recurso en línea (XI, 243 páginas) _b42 ilustraciones, 32 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v2211
505	0		_aImproving E. coli Bactofection by Expression of Bacteriophage ΦX174 Gene E -- Improved DNA Delivery Efficiency of Bacterial Vectors by Co-Delivery with Exogenous Lipid and Antimicrobial Reagents -- Visualization of Bacteria-Mediated Gene Delivery Using High Resolution Electron and Confocal Microscopy -- Assessing Nucleic Acid:Cationic Nanoparticle Interaction for Gene Delivery -- Catanionic Hybrid Lipid Nanovesicles for Improved Bioavailability and Efficacy of Chemotherapeutic Drugs -- Magnetic Nanoparticles as a Carrier of dsRNA for Gene Therapy -- Generation of Functional Insulin-Producing Cells from Mouse Embryonic Stem Cells through Protein Transduction of Transcription Factors -- Cardiac Targeting Peptide: From Identification to Validation to Mechanism of Transduction -- Lactoferricin-Derived L5a Cell-Penetrating Peptide for Delivery of DNA into Cells -- Delivery of Peptide Nucleic Acids Using an Argininocalix[4]arene as Vector -- Cell-Derived Nanovesicles as Exosome-Mimetics for Drug Delivery Purposes: Uses and Recommendations -- Hydrogel Beads of Natural Polymers as a Potential Vehicle for Colon-Targeted Drug Delivery -- Scaffold-Based Delivery of CRISPR/Cas9 Ribonucleoproteins for Genome Editing -- Correction of Hemoglobin E/Beta-Thalassemia Patient-Derived iPSCs Using CRISPR/Cas9 -- CRISPR/Cas9-Mediated GFP Reporter Knock-In in K562 and Raji Cell Lines for Tracking Immune Cell Killing Assay -- Shotgun Proteomics and Mass Spectrometry as a Tool for Protein Identification and Profiling of Bio-Carrier-Based Therapeutics on Human Cancer Cells.
520			_aThis detailed book explores techniques used in research efforts to adopt and combine the best capabilities from natural and artificial vector systems to assemble improved delivery technologies aimed for specific applications. The collection discusses vectors other than the traditional viral and non-viral systems and explores ideas for blending the best features of bacteria, nanoparticles, peptides, and hybrid systems for the delivery of biomaterials into cells, as well as a chapter on the application of shotgun proteomics and mass spectrometry as a tool to analyze the proteomic profile changes in cells that result from these interventions. Written in the highly successful Methods in Molecular Biology format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Bio-Carrier Vectors: Methods and Protocols serves as an ideal guide for researchers working toward harnessing the power of multiple vectors in gene and drug delivery.
988			_aSpringer_Protocols_2021
650		7	_2embne _9667667 _aSistemas de liberación de fármacos _vManuales de laboratorio
776	0	8	_iPrinted edition: _z9781071609422
776	0	8	_iPrinted edition: _z9781071609446
776	0	8	_iPrinted edition: _z9781071609453
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-0716-0943-9 _zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
942			_2lcc _cLE
998			_b08/2023 _dz _eu _zSI