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020 _a9781493925728
024 7 _a10.1007/978-1-4939-2572-8
_2doi
040 _aES-MaUEC
_bspa
_cES-MaUEC
_dES-MaUEC
050 4 _aQP111.4
_b2015 EB
245 0 0 _aCardiomyocytes :
_bMethods and Protocols
_cedited by Gary R. Skuse, Maureen C. Ferran
250 _a1st edition 2015
264 1 _aNew York, NY
_bSpringer International Publishing
_c2015
300 _a1 recurso en línea (X, 216 páginas)
_b36 ilustraciones, 28 ilustraciones a color
336 _atexto
_btxt
_2rdacontent
337 _aelectrónico
_bc
_2rdamedia
338 _arecurso electrónico
_bcr
_2rdacarrier
347 _aarchivo de texto
_bPDF
490 0 _aMethods in Molecular Biology
_x1940-6029
_v1299
505 0 _aGenerating Primary Cultures of Murine Cardiac Myocytes and Cardiac Fibroblasts to Study Viral Myocarditis -- Enrichment of Cardiomyocytes in Primary Cultures of Murine Neonatal Hearts -- Deep Sequencing of Cardiac MicroRNA-mRNA Interactomes in Clinical and Experimental Cardiomyopathy -- Next Generation Sequencing Technology in the Genetics of Cardiovascular Disease -- Computational Cardiac Electrophysiology: Implementing Mathematical Models of Cardiomyocytes to Simulate Action Potentials of the Heart -- Methods of Myofibrillogenesis Modeling -- Using the Mechanical Bidomain Model to Analyze the Biomechanical Behavior of Cardiomyocytes -- Fabrication of a Myocardial Patch with Cells Differentiated from Human Induced-Pluripotent Stem Cells -- Efficient Differentiation of Cardiomyocytes from Human Pluripotent Stem Cells with Growth Factors -- Isolation, Culturing, and Characterization of Cardiac Muscle Cells from Non-Human Primate Heart Tissue -- Mouse Embryonic Stem Cell-Derived Cardiac Myocytes in a Cell Culture Dish -- Cryopreservation of Neonatal Cardiomyocytes -- Evaluation of Sarcomeric Organization in Human Pluripotent Stem Cell-Derived Cardiomyocytes -- Electrotonic Coupled Metabolic Purification of Chick Cardiomyocytes -- Gene Transfer into Cardiac Myocytes -- Analysis of 4D Myocardial Wall Motion During Early Stages of Chick Heart Development.
520 _aThis volume has been assembled for scientists interested in basic and applied biomedical research directed toward understanding the development, genetics, and function of cardiomyocytes.  The methods and protocols contained within it address cell culture techniques, cardiomyocyte differentiation and redifferentiation, experimental induction of cardiomyopathies, introducing genes into cardiomyocytes, genomic approaches to the understanding cardiomyocytes, cryopreservation of neonatal cardiomyocytes, and modeling of cardiomyocyte function. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Practical and current, Cardiomyocytes: Methods and Protocols explores complimentary areas of cardiomyocyte science that, taken together, can inform individuals with a broad range of interests.
988 _aSpringer_Protocols_2015
650 7 _2embne
_9138127
_aCorazón
_vManuales de laboratorio
776 0 8 _iPrinted edition:
_z9781493925735
776 0 8 _iPrinted edition:
_z9781493925711
776 0 8 _iPrinted edition:
_z9781493947546
856 4 0 _uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-4939-2572-8
_zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
942 _2lcc
_cLE
998 _b07/2023
_dz
_eu
_zSI