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020			_a9781071616017
024	7		_a10.1007/978-1-0716-1601-7 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC _dES-MaUEC
050		4	_aQP363.3 _b2021 EB
245	0	0	_aNeural Reprogramming : _bMethods and Protocols _cedited by Henrik Ahlenius
250			_a1st edition 2021
264		1	_aNew York, NY _bSpringer International Publising _c2021
300			_a1 recurso en línea (XI, 263 páginas) _b46 ilustraciones, 43 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v2352
505	0		_aIsolation and Neuronal Reprogramming of Mouse Embryonic Fibroblasts -- Direct In Vitro Reprogramming of Astrocytes into Induced Neurons -- Direct Cell Reprogramming of Mouse Fibroblast into Functional Astrocytes Using Lentiviral Overexpression of the Transcription Factors NFIA, NFIB, and SOX9 -- Direct Reprogramming of Fibroblasts to Astrocytes Using Small Molecules -- Bcl-2-Assisted Reprogramming of Mouse Astrocytes and Human Fibroblasts into Induced Neurons -- Direct Conversion of Human Fibroblasts to Induced Neurons -- Generation of Induced Dopaminergic Neurons from Human Fetal Fibroblasts -- Direct Differentiation of Functional Neurons from Human Pluripotent Stem Cells (hPSCs) -- Generation of Motor Neurons from Human ESCs/iPSCs Using Sendai Virus Vectors -- Transcription Factor Programming of Human Pluripotent Stem Cells to Functionally Mature Astrocytes for Monocultures and Cocultures with Neurons -- Single Transcription Factor-Based Differentiation Allowing Fast and Efficient Oligodendrocyte Generation via SOX10 Overexpression -- Transcriptional Profiling During Neural Conversion -- Functional Assessment of Direct Reprogrammed Neurons In Vitro and In Vivo -- Neurotransmitter Release of Reprogrammed Cells Using Electrochemical Detection Methods -- Combining Cell Fate Reprogramming and Protein Engineering to Study Transcription Factor Functions -- CRISPR/Cas9-Genome Engineering in Human Pluripotent Stem Cells for Modeling of Neurological Disorders -- Derivation of Adult Human Cortical Organotypic Slice Cultures for Co-Culture with Reprogrammed Neuronal Cells.
520			_aThis detailed book brings together a number of state-of-the-art protocols to generate different types of neural cells through the use of reprogramming technologies. Additionally, the volume explores different aspects of functional evaluation and applications of reprogrammed neural cells as well as in silico methods to aid reprogramming efforts. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Neural Reprogramming: Methods and Protocols provides ample experimental experience and guidance for anyone, be it experienced researcher or beginner, to generate, validate, and apply reprogrammed neural cells in their research.
988			_aSpringer_Protocols_2021
650		7	_2embne _9144348 _aNeuronas _vManuales de laboratorio
650		7	_2embne _9158907 _aNeurociencias _vManuales de laboratorio
776	0	8	_iPrinted edition: _z9781071616000
776	0	8	_iPrinted edition: _z9781071616024
776	0	8	_iPrinted edition: _z9781071616031
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-0716-1601-7 _zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
942			_2lcc _cLE
998			_b06/2023 _dz _eu _zSI