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003			ES-MaUEC
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008			210724s2021 xxu| o |||| 0|eng d
020			_a9781071614846
024	7		_a10.1007/978-1-0716-1484-6 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC _dES-MaUEC
050		4	_aQH588 .S83 _b2021 EB
245	0	0	_aPluripotent Stem-Cell Derived Cardiomyocytes _cedited by Yoshinori Yoshida
250			_a1st edition 2021
264		1	_aNew York, NY _bSpringer International Publising _c2021
300			_a1 recurso en línea (XIV, 304 páginas) _b98 ilustraciones, 73 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v2320
505	0		_aMaking Cardiomyocytes from Pluripotent Stem Cells -- A method for Large-Scale Cardiac Differentiation, Purification, and Cardiac Spheroid Production of Human Induced Pluripotent Stem Cells -- Large-scale Differentiation of Stem Cell-Derived Cardiomyocytes by Stirring-Type Suspension Culture -- Efficient Method to Dissociate Induced Pluripotent Stem Cell-derived Cardiomyocyte Aggregates into Single Cells -- Isolation of Cardiomyocytes Derived from Human Pluripotent Stem Cells using miRNA switches -- Fabrication of Cardiac Constructs using bio-3D Printer -- Fabrication of Thick and Anisotropic Cardiac Tissue on Nanofibrous Substrate for Repairing Infarcted Myocardium -- Construction of Three-Dimensional Cardiac Tissues Using Layer by Layer Method -- Generation of Cylindrical Engineered Cardiac Tissues from human iPS Cell-derived Cardiovascular Cell Lineages -- Protocol for Morphological and Functional Phenotype Analysis of hiPS-derived Cardiomyocytes -- Application of FluoVolt Membrane Potential Dye for Induced Pluripotent Stem Cell-derived Cardiac Single Cells and Monolayers Differentiated via Embryoid Bodies -- Multi-Electrode Array Assays Using Human Induced Pluripotent Stem Cell-Derived Cardiomyocytes -- Electrophysiological Analysis of hiPSC-derived Cardiomyocytes Using a Patch-Clamp Technique -- Characterization of Ventricular and Atrial Cardiomyocyte Subtypes from Human Induced Pluripotent Stem Cells -- Assessment of Contractility in Human iPS Cell-Derived Cardiomyocytes Using Motion Vector Analysis -- Contractile Force Measurement of Engineered Cardiac Tissues Derived from Human iPS Cells -- A Method for Contraction Force Measurement of iPSC Derived Engineered Cardiac Tissues -- Single-cardiomyocyte RNA Sequencing to Dissect the Molecular Pathophysiology of the Heart -- RNA-sequencing Analysis of Differentially Expressed Genes in Human iPSC-derived Cardiomyocytes -- Analysis of Transcriptional Profiling of Chamber-specific Human Cardiac Myocytes Derived from Pluripotent Stem Cells -- Genome Editing in Human Induced Pluripotent Stem Cells (hiPSCs) -- Generation of Efficient Knock-in Mouse and Human Pluripotent Stem Cells Using CRISPR-Cas9 -- CRISPRi/a Screening with Human iPSCs -- Transplantation of Human Induced Pluripotent Stem Cell-derived Cardiomyocytes in a Mouse Myocardial Infarction Model -- Transplantation of Pluripotent Stem Cell-derived Cardiomyocytes into a Myocardial Infarction Model of Cynomolgus Monkey. .
520			_aThis volume provides methodologies for ES and iPS cell technology on the study of cardiovascular diseases. Chapters guide readers through protocols on cardiomyocyte generation from pluripotent stem cells, physiological measurements, bioinformatic analysis, gene editing technology, and cell transplantation studies. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Pluripotent Stem-Cell Derived Cardiomyocytes aims to help researchers set up experiments using pluripotent stem cell-derived cardiac cells.
988			_aSpringer_Protocols_2021
650		7	_2embne _9160528 _aCélulas madre _vManuales de laboratorio
776	0	8	_iPrinted edition: _z9781071614839
776	0	8	_iPrinted edition: _z9781071614853
776	0	8	_iPrinted edition: _z9781071614860
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-0716-1484-6 _zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
942			_2lcc _cLE
998			_b06/2023 _dz _eu _zSI