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008			210401s2021 xxu| o |||| 0|eng d
020			_a9781071613863
024	7		_a10.1007/978-1-0716-1386-3 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC _dES-MaUEC
050		4	_aQP623 _b2021 EB
245	0	0	_aSmall Non-Coding RNAs : _bMethods and Protocols _cedited by Mathieu Rederstorff
250			_a2nd edition 2021
264		1	_aNew York, NY _bSpringer International Publising _c2021
300			_a1 recurso en línea (X, 270 páginas) _b66 ilustraciones, 48 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v2300
505	0		_aRegulatory Non-coding RNAs: An Overview -- RNA Precipitation -- Quantitative and Qualitative Assessment of Small RNA Preparations -- Non-coding RNA Silencing in Mammalian Cells by Antisense LNA GapmeRs Transfection -- Northern Blot Detection of Tiny RNAs -- Stem-loop qRT-PCR Based Quantification of miRNAs -- Detection and Labeling of Small Non-coding RNAs by Splinted Ligation -- Fluorescence In Situ Hybridization Of Small Non-coding RNAs -- Using native RIP, UV-CLIP or fCLIP to Address Protein-RNA Interactions In Vivo -- Purification of RiboNucleoProtein Particles by MS2-MBP Affinity Chromatography -- Study of genome-wide occupancy of long non-coding RNAs using Chromatin Isolation by RNA Purification (ChIRP) -- Gene Reporter Assays to Study miRNA Function -- CRISPR/Cas9 System to Knock-Down microRNA In Vitro and In Vivo -- Microarray Analysis of Whole-Transcriptome RNAs including Non-Coding RNAs -- Isolation, Extraction, and Deep-Sequencing Analysis Of Extracellular RNAs (exRNAs) from Human Plasma -- Analysis of the RNA and protein complexome by Grad-seq -- µIVC-Seq: A Method for Ultrahigh-throughput Development and Functional Characterization of Small RNAs -- RNA Secondary Structure Study by Chemical Probing Methods using DMS and CMCT -- Application of NMR Spectroscopy to Determine the 3D structure of Small Non-coding RNAs.
520			_aThis second edition provides new and updated chapters detailing methods tackling all aspects of small non-coding RNAs biology. Chapters guide readers through customized dedicated protocols and technologies that will help decipher the numerous functions of small non-coding RNAs. Written in the highly successful Methods of Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols and key tips on troubleshooting and avoiding known pitfalls. Instructive and practical, Small Non-Coding RNAs: Methods and Protocols, Second Edition aims to be a valuable help and of great interest to a large number of researchers working in the field of RNA or willing to develop RNA studies.
988			_aSpringer_Protocols_2021
650		7	_2embne _9144940 _aARN _vManuales de laboratorio
776	0	8	_iPrinted edition: _z9781071613856
776	0	8	_iPrinted edition: _z9781071613870
776	0	8	_iPrinted edition: _z9781071613887
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-0716-1386-3 _zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
942			_2lcc _cLE
998			_b06/2023 _dz _eu _zSI